tipo de financiamento |
European Comission |
programa |
FP7-IDEAS-ERC |
acrónimo/
referência |
IGYPURTECH |
referência alternative |
337753 |
grupo(s) de investigação |
5 - materiais biomédicos e biomiméticos; |
departamento |
Chemistry (DQ) |
período de execução |
2014-02-01 - 2019-01-31 (
60 Meses )
|
resumo/
palavras-chave |
With the emergence of antibiotic-resistant pathogens the development of antigen-specific antibodies for use in passive immunotherapy is, nowadays, a major concern in human society. Despite the most focused mammal antibodies, antibodies obtained from egg yolk of immunized hens, immunoglobulin Y (IgY), are an alternative option that can be obtained in higher titres by non-stressful and non-invasive methods. This large amount of available antibodies opens the door for a new kind of cheaper biopharmaceuticals. However, the production cost of high-quality IgY for large-scale applications remains higher than other drug therapies due to the lack of an efficient purification method. The search of new purification platforms is thus a vital demand to which liquid-liquid extraction using aqueous biphasic systems (ABS) could be the answer. Besides the conventional polymer-based systems, highly viscous and with a limited polarity/affinity range, a recent type of ABS composed of ionic liquids (ILs) may be employed. ILs are usually classified as “green solvents” due to their negligible vapour pressure. Yet, the major advantage of IL-based ABS relies on the possibility of tailoring their phases’ polarities aiming at extracting a target biomolecule. A proper manipulation of the system constituents and respective composition allows the pre-concentration, complete extraction, or purification of the most diverse biomolecules. This research project addresses the development of a new technique for the extraction and purification of IgY from egg yolk using IL-based ABS. The proposed plan contemplates the optimization of purification systems at the laboratory scale and their use in countercurrent chromatography to achieve a simple, cost-effective and scalable process. The success of this project and its scalability to an industrial level certainly will allow the production of cheaper antibodies with a long-term impact in human healthcare."
|
coordenador
/ip local |
Mara Guadalupe Freire Martins |
ciceco status |
Coordenador |
instituição proponente |
Universidade de Aveiro (UA) |
instituições participante(s) |
no
|
participação industrial |
Não |
parceiro(s) internacional |
Não |
orçamento total |
1.386.020€
|
orçamento ciceco |
1.386.020€
|
centro de custos |
3.89.186 |
link |
http://cordis.europa.eu/project/rcn/111015_en.html
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Synthesis and characterization of analogues of glycine-betaine ionic liquids and their use in the formation of aqueous biphasic systemsPereira, MM; Pedro, SN; Gomes, J; Sintra, TE; Ventura, SPM; Coutinho, JAP; Freire, MG; Mohamadou, A 2019, FLUID PHASE EQUILIBRIA, 494, 239-245.
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Can cholinium chloride form eutectic solvents with organic chloride-based salts?Abranches, DO; Silva, LP; Martins, MAR; Fernandez, L; Pinho, SP; Coutinho, JAP 2019, FLUID PHASE EQUILIBRIA, 493, 120-126.
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Odd-Even Effect in the Formation and Extraction Performance of Ionic-Liquid-Based Aqueous Biphasic SystemsBelchior, DCV; Almeida, MR; Sintra, TE; Ventura, SPM; Duarte, IF; Freire, MG 2019, INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 58, 19, 8323-8331.
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Solvatochromism as a new tool to distinguish structurally similar compoundsMadeira, PP; Loureiro, JA; Freire, MG; Coutinho, JAP 2019, JOURNAL OF MOLECULAR LIQUIDS, 274, 740-745.
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Stimuli responsive ion gels based on polysaccharides and other polymers prepared using ionic liquids and deep eutectic solventsPrasad, K; Mondal, D; Sharma, M; Freire, MG; Mukesh, C; Bhatt, J 2018, CARBOHYDRATE POLYMERS, 180, 328-336.
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Cloud Point Extraction of Chlorophylls from Spinach Leaves Using Aqueous Solutions of Nonionic SurfactantsLeite, AC; Ferreira, AM; Morais, ES; Khan, I; Freire, MG; Coutinho, JAP 2018, ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 6, 1, 590-599.
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Mechanisms ruling the partition of solutes in ionic-liquid-based aqueous biphasic systems - the multiple effects of ionic liquidsPassos, H; Dinis, TBV; Capela, EV; Quental, MV; Gomes, J; Resende, J; Madeira, PP; Freire, MG; Coutinho, JAP 2018, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 20, 13, 8411-8422.
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Economic evaluation of the primary recovery of tetracycline with traditional and novel aqueous two-phase systemsTorres-Acosta, MA; Pereira, JFB; Freire, MG; Aguilar-Yanez, JM; Coutinho, JAP; Titchener-Hooker, NJ; Rito-Palomares, M 2018, SEPARATION AND PURIFICATION TECHNOLOGY, 203, 178-184.
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Hydrogen bond basicity of ionic liquids and molar entropy of hydration of salts as major descriptors in the formation of aqueous biphasic systemsPassos, H; Dinis, TBV; Claudio, AFM; Freire, MG; Coutinho, JAP 2018, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 20, 20, 14234-14241.
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Odd-even effect on the formation of aqueous biphasic systems formed by 1-alkyl-3-methylimidazolium chloride ionic liquids and saltsBelchior, DCV; Sintra, TE; Carvalho, PJ; Soromenho, MRC; Esperanca, JMSS; Ventura, SPM; Rogers, RD; Coutinho, JAP; Freire, MG 2018, JOURNAL OF CHEMICAL PHYSICS, 148, 19,
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An integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible aqueous biphasic systemsFerreira, AM; Passos, H; Okafuji, A; Tavares, APM; Ohno, H; Freire, MG; Coutinho, JAP 2018, GREEN CHEMISTRY, 20, 6, 1218-1223.
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Deep Eutectic Solvent Aqueous Solutions as Efficient Media for the Solubilization of Hardwood XylansMorais, ES; Mendonca, PV; Coelho, JFJ; Freire, MG; Freire, CSR; Coutinho, JP; Silvestre, AJD 2018, CHEMSUSCHEM, 11, 4, 753-762.
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Valorization of olive tree leaves: Extraction of oleanolic acid using aqueous solutions of surface-active ionic liquidsClaudio, AFM; Cognigni, A; de Faria, ELP; Silvestre, AJD; Zirbs, R; Freire, MG; Bica, K 2018, SEPARATION AND PURIFICATION TECHNOLOGY, 204, 30-37.
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Enhanced separation performance of aqueous biphasic systems formed by carbohydrates and tetraalkylphosphonium- or tetraalkylammonium-based ionic liquidsQuental, MV; Pereira, MM; Ferreira, AM; Pedro, SN; Shahriari, S; Mohamadou, A; Coutinho, JAP; Freire, MG 2018, GREEN CHEMISTRY, 20, 13, 2978-2983.
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Pioneering Use of Ionic Liquid-Based Aqueous Biphasic Systems as Membrane-Free BatteriesNavalpotro, P; Neves, CMSS; Palma, J; Freire, MG; Coutinho, JAP; Marcilla, R 2018, ADVANCED SCIENCE, 5, 10,
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Switchable (pH-driven) aqueous biphasic systems formed by ionic liquids as integrated production-separation platformsFerreira, AM; Claudio, AFM; Valega, M; Domingues, FMJ; Silvestre, AJD; Rogers, RD; Coutinho, JAP; Freire, MG 2017, GREEN CHEMISTRY, 19, 12, 2768-2773.
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Solvatochromic parameters of deep eutectic solvents formed by ammonium-based salts and carboxylic acidsTeles, ARR; Capela, EV; Carmo, RS; Coutinho, JAP; Silvestre, AJD; Freire, MG 2017, FLUID PHASE EQUILIBRIA, 448, 15-21.
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Alternative probe for the determination of the hydrogen-bond acidity of ionic liquids and their aqueous solutionsMadeira, PP; Passos, H; Gomes, J; Coutinho, JAP; Freire, MG 2017, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 19, 18, 11011-11016.
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Designing the thermal behaviour of aqueous biphasic systems composed of ammonium-based zwitterionsFerreira, AM; Passos, H; Okafuji, A; Freire, MG; Coutinho, JAP; Ohno, H 2017, GREEN CHEMISTRY, 19, 17, 4012-4016.
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Improved monitoring of aqueous samples by the preconcentration of active pharmaceutical ingredients using ionic-liquid-based systemsAlmeida, HFD; Freire, MG; Marrucho, IM 2017, GREEN CHEMISTRY, 19, 19, 4651-4659.
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