Extraction of tetracycline from fermentation broth using aqueous two-phase systems composed of polyethylene glycol and cholinium-based salts
authors Pereira, JFB; Vicente, F; Santos-Ebinuma, VC; Araujo, JM; Pessoa, A; Freire, MG; Coutinho, JAP
nationality International
journal PROCESS BIOCHEMISTRY
author keywords Tetracycline; Streptomyces aureofaciens; Extraction; Aqueous two-phase systems; Cholinium-based salts
keywords BIPHASIC SYSTEMS; IONIC LIQUIDS; HYDROCHLORIDE FORMS; HOFMEISTER SERIES; ANTIBIOTICS; CHROMATOGRAPHY; CIPROFLOXACIN; SOLUBILITY
abstract Aiming at developing not only cheaper but also biocompatible and sustainable extraction and purification processes for antibiotics, in this work it was evaluated the ability of aqueous two-phase systems (ATPS) composed of polyethylene glycol (PEG) and cholinium-based salts to extract tetracycline from the fermented broth of Streptomyces aureofaciens. Conventional polymer/salt and salt/salt ATPS were also studied for comparison purposes. The novel systems here proposed are able to extract tetracycline directly from the fermentation broth with extraction efficiencies higher than 80%. A tailored extraction ability of these systems can also be achieved, with preferential extractions either for the polymer- or salt-rich phases, and which further depend on the cholinium-based salt employed. The gathered results support the applicability of biocompatible ATPS in the extraction of antibiotics from complex matrices and can be envisaged as valuable platforms to be applied at the industrial level by pharmaceutical companies. (c) 2013 Elsevier Ltd. All rights reserved.
publisher ELSEVIER SCI LTD
issn 1359-5113
year published 2013
volume 48
issue 4
beginning page 716
ending page 722
digital object identifier (doi) 10.1016/j.procbio.2013.02.025
web of science category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical
subject category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering
unique article identifier WOS:000320413900024
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