Magnetic chelating nanoprobes for enrichment and selective recovery of metalloproteases from human saliva
authors Oliveira-Silva, R; da Costa, JP; Vitorino, R; Daniel-da-Silva, AL
nationality International
journal JOURNAL OF MATERIALS CHEMISTRY B
keywords CP/MAS NMR INVESTIGATIONS; MATRIX METALLOPROTEINASES; MASS-SPECTROMETRY; SERINE PROTEASES; PROTEIN; NANOPARTICLES; SEPARATION; PARTICLES; GEL; IDENTIFICATION
abstract New magnetic nanoprobes based on surface-functionalized magnetite particles were synthesized and used to selectively capture metalloproteases from human saliva samples. In addition to their high specific surface area, these nanoparticles have metal ion chelating moieties grafted on their surface by the reaction with the organosilane EDTA-TMS ((N-(trimethoxysilylpropyl)-ethylenediaminetriacetate trisodium salt). The most distinct feature of these particles is their capability to selectively recover metalloproteases even in highly diluted saliva samples. The high affinity of these materials for metalloproteases was attributed to the formation of chelates between the chelating moieties and the metal ions of metalloproteases. These magnetic particles exhibited high colloidal stability in the biological standard buffers MES, HEPES, TEAB and Tris-HCl, within a significant pH range (pH 5-9) and, due to their magnetic features, enabled a fast separation of the metalloproteases from the medium by simply applying an external magnetic field. Thus, these materials were proven to be valuable probes for the selective enrichment and rapid recovery of metalloproteases from human saliva, particularly when dealing with trace amounts of material.
publisher ROYAL SOC CHEMISTRY
issn 2050-750X
year published 2015
volume 3
issue 2
beginning page 238
ending page 249
digital object identifier (doi) 10.1039/c4tb01189a
web of science category Materials Science, Biomaterials
subject category Materials Science
unique article identifier WOS:000346061000010
  ciceco authors
  impact metrics
journal analysis (jcr 2017):
journal impact factor 4.776
5 year journal impact factor 4.959
category normalized journal impact factor percentile 77.273
dimensions (citation analysis):
altmetrics (social interaction):



 


Sponsors

1suponsers_list_ciceco.jpg