Enzymatic synthesis of poly(catechin)-antibiotic conjugates: an antimicrobial approach for indwelling catheters
authors Goncalves, I; Abreu, AS; Matama, T; Ribeiro, A; Gomes, AC; Silva, C; Cavaco-Paulo, A
journal APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
author keywords Laccase; Catechin; Trimethoprim; Sulfamethoxazole; Antimicrobial; Catheters
keywords BACTERIAL OUTER-MEMBRANE; STAPHYLOCOCCUS-AUREUS; ANTIBACTERIAL PROPERTIES; BIOFILM RESISTANCE; TRIMETHOPRIM; ANTIBIOTICS; POLYPHENOLS; FLAVONOIDS; COMBINATION; MECHANISMS
abstract Biofilm formation in urinary indwelling catheters is one of the most critical issues that patients face. Catheters were coated with poly(catechin)-antibiotic conjugates with enhanced antimicrobial properties. Catechin was conjugated with two antibiotics, namely trimethoprim (TMP) and sulfamethoxazole (SMZ) via activation with N,N'-disuccinimidyl carbonate (DSC) and subsequent coupling to molecules containing alpha-amine moieties. Silicone and polyurethane catheters were functionalized in situ through laccase oxidation of catechin-antibiotic conjugates. Four antimicrobial coatings were produced, namely with poly(catechin), poly(catechin)-TMP, poly(catechin)-SMZ and poly(catechin)-TMP-SMZ. The bacterial adhesion reduction was tested on the functionalized devices using gram-negative and gram-positive strains. The most significant reduction in adhesion was observed with poly(catechin)-TMP (gram-negative -85 % and gram-positive -87 %) and with poly(catechin)-TMP-SMZ (gram-negative -85 % and gram-positive -91 %). The cytotoxicity to mammalian cells was tested by indirect contact for 5 days and revealed that all the tested coatings supported more than 90 % of viable cells. A promising approach for the increase of the indwelling catheter lifespan was developed aiming to reduce catheter-associated chronic infections.
publisher SPRINGER
issn 0175-7598
year published 2015
volume 99
issue 2
beginning page 637
ending page 651
digital object identifier (doi) 10.1007/s00253-014-6128-2
web of science category Biotechnology & Applied Microbiology
subject category Biotechnology & Applied Microbiology
unique article identifier WOS:000348770900009
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journal analysis (jcr 2017):
journal impact factor 3.340
5 year journal impact factor 3.602
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