Antimicrobial lubricant formulations containing poly(hydroxybenzene)-trimethoprim conjugates synthesized by tyrosinase
authors Goncalves, I; Botelho, CM; Teixeira, A; Abreu, AS; Hilliou, L; Silva, C; Cavaco-Paulo, A
journal APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
author keywords Methylparaben; Trimethoprim; Tyrosinase; Poly(hydroxybenzene); Conjugates; Antimicrobial
keywords CATHETER-RELATED INFECTION; URINARY-TRACT INFECTIONS; AQUEOUS-SOLUTIONS; METHYL PARABEN; ACID PARABENS; HYDROXYETHYLCELLULOSE; PREVENTION; PRODUCTS; HEALTH; DERIVATIVES
abstract Poly(hydroxybenzene)-trimethoprim conjugates were prepared using methylparaben as substrate of the oxidative enzyme tyrosinase. MALDI-TOF MS analysis showed that the enzymatic oxidation of methylparaben alone leads to the poly(hydroxybenzene) formation. In the presence of trimethoprim, the methylparaben tyrosinase oxidation leads poly(hydroxybenzene)-trimethoprim conjugates. All of these compounds were incorporated into lubricant hydroxyethyl cellulose/glycerol mixtures. Poly(hydroxybenzene)-trimethoprim conjugates were the most effective phenolic structures against the bacterial growth reducing by 96 and 97 % of Escherichia coli and Staphylococcus epidermidis suspensions, respectively (after 24 h). A novel enzymatic strategy to produce antimicrobial poly(hydroxybenzene)-antibiotic conjugates is proposed here for a wide range of applications on the biomedical field.
publisher SPRINGER
issn 0175-7598
year published 2015
volume 99
issue 10
beginning page 4225
ending page 4235
digital object identifier (doi) 10.1007/s00253-015-6509-1
web of science category Biotechnology & Applied Microbiology
subject category Biotechnology & Applied Microbiology
unique article identifier WOS:000353826200007
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