Complex Effect of Hydroxyapatite Nanoparticles on the Differentiation and Functional Activity of Human Pre-Osteoclastic Cells
authors Costa-Rodrigues, J; Silva, A; Santos, C; Almeida, MM; Costa, ME; Fernandes, MH
nationality International
journal JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
author keywords Hydroxyapatite Nanoparticles; Osteoclastogenesis; Peripheral Blood Mononuclear Cells; Bone Resorption; Cell Differentiation
keywords MESENCHYMAL STEM-CELLS; IN-VITRO; NANOPHASE HYDROXYAPATITE; COLLOIDAL GELS; BONE; PARTICLES; PROLIFERATION; NANO; CYTOTOXICITY; EXPRESSION
abstract Nanosized hydroxyapatite (HA) is a promising material in clinical applications targeting the bone tissue. NanoHA is able to modulate bone cellular events, which accounts for its potential utility, but also raises safety concerns regarding the maintenance of the bone homeostasis. This work analyses the effects of HA nanoparticles (HAnp) on osteoclastic differentiation and activity, an issue that has been barely addressed. Rod-like HAnp, produced by a hydrothermal precipitation method, were tested on peripheral blood mononuclear cells (PBMC), which contains the CD14+ osteoclastic precursors, in unstimulated or osteoclastogenic-induced conditions. HAnp were added at three time-points during the osteoclastic differentiation pathway, and cell response was evaluated for osteoclastic related parameters. Results showed that HAnp modulated the differentiation and function of osteoclastic cells in a dose- and time-dependent manner. In addition, the effects were dependent on the stage of osteoclastic differentiation. In unstimulated PBMC, HAnp significantly increased osteoclastogenesis, leading to the formation of mature osteoclasts, as evident by the significant increase of TRAP activity, number of TRAP-positive multinucleated cells, osteoclastic gene expression and resorbing ability. However, in a population of mature osteoclasts (formed in osteoclastogenic-induced PBMC cultures), HAnp caused a dose-dependent decrease on the osteoclastic-related parameters. These results highlight the complex effects of HAnp in osteoclastic differentiation and activity, and suggest the possibility of HAnp to modulate/disrupt osteoclastic behavior, with eventual imbalances in the bone metabolism. This should be carefully considered in bone-related and other established and prospective biomedical applications of HAnp.
publisher AMER SCIENTIFIC PUBLISHERS
issn 1550-7033
year published 2014
volume 10
issue 12
beginning page 3590
ending page 3600
digital object identifier (doi) 10.1166/jbn.2014.1873
web of science category Nanoscience & Nanotechnology; Materials Science, Biomaterials
subject category Science & Technology - Other Topics; Materials Science
unique article identifier WOS:000342793600008
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journal analysis (jcr 2017):
journal impact factor 5.068
5 year journal impact factor 4.225
category normalized journal impact factor percentile 73.575
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