Biotinylation of optically responsive gold/polyelectrolyte nanostructures
authors Pereira, SO; Trindade, T; Barros-Timmons, A
nationality International
journal GOLD BULLETIN
author keywords Biosensor; Gold nanoparticles; Optical properties; Hybrid nanostructures; Polyelectrolytes; Biotinylation
keywords FUNCTIONALIZED GOLD-NANOPARTICLES; CORE/SHELL NANOPARTICLES; ENERGY-TRANSFER; FLUORESCENCE; AGGREGATION; ASSEMBLIES; STABILITY
abstract The development of analytical platforms incorporating Au nanoparticles (NPs) for optical biosensing requires approaches to produce optically responsive and target selective materials. In the present work, fluorescence emission and bioselectivity of Au NPs have been combined by a one-step surface functionalization using fluorescein isothiocyanate (FITC) and biotin. First, colloidal Au NPs (d= 17.7 +/- 2.7 nm) exhibiting an absorption maximum (lambda(max)) at 524.5 nm have been surface-modified with the polyelectrolytes (PE) poly(allylamine hydrochloride) (PAH) and poly(sodium styrene sulfonate) (PSS). Two strategies were then assessed for the functionalization of Au NPs: (i) functionalization of PAH with biotin and subsequent coating of the NPs and (ii) surface modification of the NPs with the PE followed by post-functionalization with biotin. The effect of the surface functionalization on the colloidal NPs was monitored by optical absorption and fluorescence emission spectroscopies, dynamic light scattering (DLS), zeta potential measurements and transmission electron microscopy (TEM). Finally, the optical response and specificity of the functionalized NPs towards avidin was evaluated by optical measurements. The results have shown that these hybrid nanostructures have potential to be used in biosensing. In particular, the aggregation of biotinylated Au assemblies associated with the biorecognition of avidin could be assessed by fluorescence emission spectroscopy.
publisher SPRINGER HEIDELBERG
issn 0017-1557
year published 2015
volume 48
issue 1-2
beginning page 3
ending page 11
digital object identifier (doi) 10.1007/s13404-014-0153-x
web of science category Chemistry, Inorganic & Nuclear; Chemistry, Physical; Materials Science, Multidisciplinary
subject category Chemistry; Materials Science
unique article identifier WOS:000360071700002
  ciceco authors
  impact metrics
times cited (wos core): 1
journal impact factor (jcr 2016): 1.638
5 year journal impact factor (jcr 2016): 2.173
category normalized journal impact factor percentile (jcr 2016): 41.179
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