Insights into the impact of silver nanoparticles on human keratinocytes metabolism through NMR metabolomics
authors Carrola, J; Bastos, V; de Oliveira, JMPF; Oliveira, H; Santos, C; Gil, AM; Duarte, IF
nationality International
journal ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
author keywords NMR spectroscopy; Metabolomics; Silver nanoparticles; Keratinocytes; Nanotoxicology
keywords IN-VITRO; SILICA NANOPARTICLES; CELL-CULTURE; LIPID RAFTS; TOXICITY; CYTOTOXICITY; NANOMATERIALS; H-1-NMR; LINE; SKIN
abstract Due to their antimicrobial properties, silver nanoparticles (AgNPs) are increasingly incorporated into consumer goods and medical products. Their potential toxicity to human cells is however a major concern, and there is a need for improved understanding of their effects on cell metabolism and function. Here, Nuclear Magnetic Resonance (NMR) metabolomics was used to investigate the metabolic profile of human epidermis keratinocytes (HaCaT cell line) exposed for 48 h to 30 nm citrate-stabilized spherical AgNPs (10 and 40 p.g/mL). Intracellular aqueous extracts, organic extracts and extracellular culture medium were analysed to provide an integrated view of the cellular metabolic response. The specific metabolite variations, highlighted through multivariate analysis and confirmed by spectral integration, suggested that HaCaT cells exposed to AgNPs displayed upregulated glutathione-based antioxidant protection, increased glutaminolysis, downregulated tricarboxylic acid (TCA) cycle activity, energy depletion and cell membrane modification. Importantly, most metabolic changes were apparent in cells exposed to a concentration of AgNPs which did not affect cell viability at significant levels, thus underlying the sensitivity of NMR metabolomics to detect early biochemical events, even in the absence of a clear cytotoxic response. It can be concluded that NMR metabolomics is an important new tool in the field of in vitro nanotoxicology. (C) 2015 Elsevier Inc. All rights reserved.
publisher ELSEVIER SCIENCE INC
issn Mar-61
year published 2016
volume 589
beginning page 53
ending page 61
digital object identifier (doi) 10.1016/j.abb.2015.08.022
web of science category Biochemistry & Molecular Biology; Biophysics
subject category Biochemistry & Molecular Biology; Biophysics
unique article identifier WOS:000368321600007

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