|
authors |
Silva, NHCS; Pinto, RJB; Freire, CSR; Marrucho, IM |
|
nationality |
International |
|
journal |
COLLOIDS AND SURFACES B-BIOINTERFACES |
|
keywords |
EGG-WHITE LYSOZYME; TRANSITION-TEMPERATURE MIXTURES; AMYLOID FIBRIL FORMATION; HUMAN SERUM-ALBUMIN; IONIC LIQUIDS; CHOLINE CHLORIDE; CARBOXYLIC-ACIDS; ALPHA-SYNUCLEIN; HEN LYSOZYME; X-RAY |
|
abstract |
Amyloid fibrils have recently gained a lot of attention due to their morphology, functionality and mechanical strength, allowing for their application in nanofiber-based materials, biosensors, bioactive membranes and tissue engineering scaffolds. The in vitro production of amyloid fibrils is still a slow process, thus hampering the massive production of nanofibers and its consequent use. This work presents a new and faster (2-3 h) fibrillation method for hen egg white lysozyme (HEWL) using a deep eutectic solvent based on cholinium chloride and acetic acid. Nanofibers with dimensions of 0.5-1 mu m in length and 0.02-0.1 mu m in thickness were obtained. Experimental variables such as temperature and pH were also studied, unveiling their influence in fibrillation time and nanofibers morphology. These results open a new scope for protein fibrillation into nanofibers with applications ranging from medicine to soft matter and nanotechnology. (C) 2016 Elsevier B.V. All rights reserved. |
|
publisher |
ELSEVIER SCIENCE BV |
|
issn |
0927-7765 |
|
year published |
2016 |
|
volume |
147 |
|
beginning page |
36 |
|
ending page |
44 |
|
digital object identifier (doi) |
10.1016/j.colsurfb.2016.07.005 |
|
web of science category |
Biophysics; Chemistry, Physical; Materials Science, Biomaterials |
|
subject category |
Biophysics; Chemistry; Materials Science |
|
unique article identifier |
WOS:000384851400005
|
