Production of lysozyme nanofibers using deep eutectic solvent aqueous solutions
authors Silva, NHCS; Pinto, RJB; Freire, CSR; Marrucho, IM
nationality International
journal COLLOIDS AND SURFACES B-BIOINTERFACES
keywords EGG-WHITE LYSOZYME; TRANSITION-TEMPERATURE MIXTURES; AMYLOID FIBRIL FORMATION; HUMAN SERUM-ALBUMIN; IONIC LIQUIDS; CHOLINE CHLORIDE; CARBOXYLIC-ACIDS; ALPHA-SYNUCLEIN; HEN LYSOZYME; X-RAY
abstract Amyloid fibrils have recently gained a lot of attention due to their morphology, functionality and mechanical strength, allowing for their application in nanofiber-based materials, biosensors, bioactive membranes and tissue engineering scaffolds. The in vitro production of amyloid fibrils is still a slow process, thus hampering the massive production of nanofibers and its consequent use. This work presents a new and faster (2-3 h) fibrillation method for hen egg white lysozyme (HEWL) using a deep eutectic solvent based on cholinium chloride and acetic acid. Nanofibers with dimensions of 0.5-1 mu m in length and 0.02-0.1 mu m in thickness were obtained. Experimental variables such as temperature and pH were also studied, unveiling their influence in fibrillation time and nanofibers morphology. These results open a new scope for protein fibrillation into nanofibers with applications ranging from medicine to soft matter and nanotechnology. (C) 2016 Elsevier B.V. All rights reserved.
publisher ELSEVIER SCIENCE BV
issn 0927-7765
year published 2016
volume 147
beginning page 36
ending page 44
digital object identifier (doi) 10.1016/j.colsurfb.2016.07.005
web of science category Biophysics; Chemistry, Physical; Materials Science, Biomaterials
subject category Biophysics; Chemistry; Materials Science
unique article identifier WOS:000384851400005
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