Effects of emerging contaminants on neurotransmission and biotransformation in marine organisms - An in vitro approach
authors Luis, LG; Barreto, A; Trindade, T; Soares, AMVM; Oliveira, M
nationality International
journal MARINE POLLUTION BULLETIN
author keywords Gold nanopartides; Human pharmaceuticals; Mixtures; Phase II biotransformation; Neurotransmission; Mytilus galloprovincialis
keywords MUSSEL MYTILUS-GALLOPROVINCIALIS; POMATOSCHISTUS-MICROPS TELEOSTEI; GLUTATHIONE S-TRANSFERASES; HEAVY-METAL INHIBITION; ZEBRAFISH DANIO-RERIO; FRESH-WATER MUSSELS; SHORT-TERM EXPOSURE; GOLD NANOPARTICLES; WASTE-WATER; ACETYLCHOLINESTERASE ACTIVITY
abstract The effects of gold (ionic form and nanopartides - AuNPs) and pharmaceuticals (carbamazepine and fluoxetine) on enzymes involved in neurotransmission (acetylcholinesterase - AChE) and biotransformation (glutathione S-transferases - GST) were assessed by their incubation with Mytilus galloprovincialis' hemolymph and subcellular fraction of gills, respectively. AuNPs did not alter enzymatic activities unlike ionic gold that inhibited AChE and GST activities at 2.5 and 0.42 mg.L-1, respectively. Carbamazepine inhibited AChE activity at 500 mg.L-1 and fluoxetine at 1000 mg.L-1. GST was inhibited by carbamazepine at 250 mg.L-1 and by fluoxetine at 125 mg.L-1. Increased AChE activity was found in simultaneous exposures to fluoxetine and bovine serum albumin coated AuNPs (BSA-AuNPs). Concerning GST, in the simultaneous exposures, AuNPs revealed protective effects against carbamazepine (citrate and polyvinylpyrrolidone coated) and fluoxetine (citrate and BSA coated) induced inhibition. However, BSA-AuNPs increased the inhibition caused by carbamazepine. AuNPs demonstrated ability to interfere with other chemicals toxicity justifying further studies. (C) 2016 Elsevier Ltd. All rights reserved.
publisher PERGAMON-ELSEVIER SCIENCE LTD
issn 0025-326X
year published 2016
volume 106
issue 01-Feb
beginning page 236
ending page 244
digital object identifier (doi) 10.1016/j.marpolbul.2016.02.064
web of science category Environmental Sciences; Marine & Freshwater Biology
subject category Environmental Sciences & Ecology; Marine & Freshwater Biology
unique article identifier WOS:000376545900041
  ciceco authors
  impact metrics
times cited (wos core): 2
journal impact factor (jcr 2016): 3.146
5 year journal impact factor (jcr 2016): 3.780
category normalized journal impact factor percentile (jcr 2016): 82.564
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