Extraction of an active enzyme by self-buffering ionic liquids: a green medium for enzymatic research
authors Gupta, BS; Taha, M; Lee, MJ
nationality International
journal RSC ADVANCES
keywords AQUEOUS BIPHASIC SYSTEMS; ALPHA-CHYMOTRYPSIN; BIOLOGICAL BUFFERS; DIRECT ELECTROCHEMISTRY; CARBON NANOTUBES; PH BUFFERS; SOLVENTS; CATALYSIS; AGGREGATION; EQUILIBRIA
abstract In this work, the extraction of a model enzyme alpha-chymotrypsin in its active conformation from an aqueous solution by using new biocompatible and self-buffering Good's buffer ionic liquids (GBILs)-based aqueous biphasic systems (ABSs) is demonstrated. The experimental liquid-liquid phase boundary data for these new GBIL-based ABSs were measured at 298.2 K. These new ionic liquids are derived from biological Good's buffers as anions and tetra-alkylammonium as cations. The buffering potential of these GBILs was confirmed by measuring their pH profiles and protonation constants (pK(a)) in aqueous solution by a potentiometric method at various temperatures. Moreover, these GBILs provide a greater stabilizing effect on the activity of alpha-chymotrypsin, as compared with the corresponding Good's buffers. Therefore, the new self-buffering GBILs may not only serve as a biocompatible medium for enzymatic research, but also avoid the use of an external buffering agent, which is often needed for pH control in the case of using a conventional ILs-based medium. Therefore, these new biocompatible GBILs are more advantageous over the conventional ILs-based media for enzymatic research.
publisher ROYAL SOC CHEMISTRY
issn 2046-2069
year published 2016
volume 6
issue 22
beginning page 18567
ending page 18576
digital object identifier (doi) 10.1039/c6ra00607h
web of science category Chemistry, Multidisciplinary
subject category Chemistry
unique article identifier WOS:000370717900082
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journal impact factor 2.936
5 year journal impact factor 3.096
category normalized journal impact factor percentile 58.772
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