The interaction of Hg2+ and trivalent ions with two new fluorescein bio-inspired dual colorimetric/fluorimetric probes

authors	Goncalves, AC; Pilla, V; Oliveira, E; Santos, SM; Capelo, JL; Dos Santos, AA; Lodeiro, C
nationality	International
journal	DALTON TRANSACTIONS
keywords	AQUEOUS-SOLUTION; METAL-IONS; MERCURY; EMISSION; SENSORS; DERIVATIVES; CHROMIUM; ALUMINUM; SYSTEMS; CHEMODOSIMETERS
abstract	Two new luminescent compounds containing fluorescein-amino acid units have been designed and synthesized via an ester linkage between a fluorescein ethyl ester and Boc-Ser(TBDMS)-OH or Boc-Cys(4-MeBzl-OH, and their photophysical properties have been explored. The optical response of both compounds (2 and 3) towards the metal ions Na+, K+, Hg+, Ag+, Ca2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+, Pb2+, Hg2+, Al3+, Fe3+, Ga3+ and Cr3+ was investigated in pure acetonitrile and in acetonitrile/water mixtures. A strong CHEF (Chelation-Enhanced Fluorescence) effect was observed with all the trivalent metals and Hg2+ ions in both solvents. UV-vis absorption, steady state and time resolved emission spectroscopy methods were employed. The results show the formation of mononuclear complexes with Al3+, Fe3+, Ga3+, Cr3+, and Hg2+. Theoretical calculation using Density Functional Theory was performed in order to obtain atomistic insights into the coordination geometry of Al3+ and Hg2+ to the fluorescein 3, which is in accordance with the experimental stoichiometry results obtained in the Job's plot method. Among the active cations, the minimum detectable amount is under 1 mu M for most of the cases in both absorption and fluorescence spectroscopy methods.
publisher	ROYAL SOC CHEMISTRY
issn	1477-9226
year published	2016
volume	45
issue	23
beginning page	9513
ending page	9522
digital object identifier (doi)	10.1039/c6dt01180b
web of science category	Chemistry, Inorganic & Nuclear
subject category	Chemistry
unique article identifier	WOS:000378583500017