Tuning cell adhesive properties via layer-by-layer assembly of chitosan CrossMark and alginate
authors Silva, JM; Garcia, JR; Reis, RL; Garcia, AJ; Mano, JF
nationality International
journal ACTA BIOMATERIALIA
author keywords Surface chemistry; Cross-linking; Cell adhesion; Fibronectin; Tissue engineering
keywords POLYELECTROLYTE MULTILAYER FILMS; ATOMIC-FORCE MICROSCOPY; IN-VITRO; BIOMEDICAL APPLICATIONS; REGENERATIVE MEDICINE; SURFACE MODIFICATION; GENIPIN; POLYSACCHARIDES; ADSORPTION; LINKING
abstract Understanding the mechanisms controlling cell-multilayer film interactions is crucial to the successful engineering of these coatings for biotechnological and biomedical applications. Herein, we present a strategy to tune the cell adhesive properties of multilayers based on marine polysaccharides with and without cross-linking and/or coating with extracellular matrix proteins. Chemical cross-linking of multi layers improved mechanical properties of the coatings but also elicited changes in surface chemistry that alter the adhesion of human umbilical vein endothelial cells. We evaluated a strategy to decouple the mechanical resistant multilayers. Addition of chitosan/alginate multilayers on top of cross-linked films decreased endothelial cell adhesion, spreading, and proliferation to similar levels as uncross-linked films. Our findings highlight the key role of surface chemistry in cell-multilayer film interactions, and these engineered nanocoatings represent a tunable model of cell adhesive and non-adhesive multilayered films. Statement of Significance Multilayered films based on marine-derived polysaccharides were obtained by layer-by-layer (LbL). Biological tests with human umbilical vein endothelial cells (HUVECs) showed the potential of these films to tailor cell adhesion, spreading and proliferation. These multilayered films promise to be versatile and tunable model of cell adhesive and non-adhesive films. (c) 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
publisher ELSEVIER SCI LTD
issn 1742-7061
year published 2017
volume 51
beginning page 279
ending page 293
digital object identifier (doi) 10.1016/Lactbio.2017.01.058
web of science category Engineering, Biomedical; Materials Science, Biomaterials
subject category Engineering; Materials Science
unique article identifier WOS:000398014000022
  ciceco authors
  impact metrics
times cited (wos core): 1
journal impact factor (jcr 2016): 6.319
5 year journal impact factor (jcr 2016): 6.804
category normalized journal impact factor percentile (jcr 2016): 96.104
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