Development of hydroxyapatite nanoparticles loaded with folic acid to induce osteoblastic differentiation
authors Santos, C; Gomes, P; Duarte, JA; Almeida, MM; Costa, MEV; Fernandes, MH
nationality International
journal INTERNATIONAL JOURNAL OF PHARMACEUTICS
author keywords Folic acid; Nanohydroxyapatite; Osteoblastic induction; Bone regeneration
keywords BONE-MINERAL DENSITY; MESENCHYMAL STEM-CELLS; NANOPHASE HYDROXYAPATITE; PLASMA HOMOCYSTEINE; CANCER-CELLS; FOLATE; VITAMIN-B-12; PARTICLES; FRACTURE; DELIVERY
abstract Recently it has been shown that folic acid can have an important role in bone regeneration. For this reason, combining a classic bone regeneration system as, hydroxyapatite, loaded with folic acid, may be an important issue to be developed. To address this issue, hydroxyapatite nanoparticles loaded with folic acid were designed as an effective bone regenerative system, to induce osteoblast differentiation and improve the bone regeneration. HapNP were prepared by a hydrothermal method that used citric acid as a tailoring agent of particles morphology and, simultaneously, had the particularly to let carboxylic pendant groups in the particle surface, which provided a platform for the immobilization of folic acid (FA), producing HapNP-FA. A comparative study among hydroxyapatite nanoparticles loaded and unloaded with folic acid in presence of human mesenchymal stem cells was performed. The results demonstrate, that nanoparticles were able to be internalized by human mesenchymal stem cells. In addition, cell proliferation and viability were not affected in a wide concentration range. Both particles induced the expression of Runx2 and the expression and activity of alkaline phosphatase. However, HapNP-FA caused a significantly higher overexpression of Runx2. The osteoblastic differentiation confirms the potential applicability of HapNP-FA in the local bone regeneration. (C) 2016 Elsevier B.V. All rights reserved.
publisher ELSEVIER SCIENCE BV
issn 0378-5173
isbn 1873-3476
year published 2017
volume 516
issue 01-Feb
beginning page 185
ending page 195
digital object identifier (doi) 10.1016/j.ijpharm.2016.11.035
web of science category Pharmacology & Pharmacy
subject category Pharmacology & Pharmacy
unique article identifier WOS:000390437300022
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journal analysis (jcr 2017):
journal impact factor 3.862
5 year journal impact factor 4.229
category normalized journal impact factor percentile 82.567
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