Diamond-Graphite Nanoplatelet Surfaces as Conductive Substrates for the Electrical Stimulation of Cell Functions
authors Santos, NF; Cicuendez, M; Holz, T; Silva, VS; Fernandes, KJS; Vila, M; Costa, FM
nationality International
journal ACS APPLIED MATERIALS & INTERFACES
author keywords nanocarbon hybrids; biocompatibility; electrical stimulation; preosteoblasts; proliferation; differentiation
keywords TISSUE ENGINEERING APPLICATIONS; PROTEIN ADSORPTION; NANOFIBROUS SCAFFOLDS; DRUG-DELIVERY; MTT ASSAY; ADHESION; PROLIFERATION; BIOMATERIALS; DIFFERENTIATION; MORPHOLOGY
abstract The nanocarbon allotropes constitute valid alternatives when designing control and actuation devices for electrically assisted tissue regeneration purposes, gathering among them important characteristics such as chemical inertness, biocompatibility, extreme mechanical properties, and, importantly, low and tailorable electrical resistivity, In this work, coatings of thin (100 nm) vertically aligned nanoplatelets composed of diamond (5 nm) and graphite were produced via a microwave plasma chemical vapor deposition (MPCVD) technique and used as substrates for electrical stimulation of MC3T3-E1 preosteoblasts. Increasing the amount of N-2 up to 14.5 vol % during growth lowers the coatings'-electrical resistivity by over 1 order of magnitude, triggers the nanoplatelet vertical growth, and leads to the higher crystalline quality of the nanographite phase. When preosteoblasts were cultured on these substrates and subjected to two consecutive, daily cycles of 3 mu A direct current stimulation, enhanded cell proliferation and metabolism were observed accompanied by high cell viability. Furthermore, in the absence of DC stimulation, alkaline phosphatase (ALP) activity is increased significantly, denoting an up-regulating effect of preosteoblastic maturation intrinsically exerted by the nanoplatelet substrates.
publisher AMER CHEMICAL SOC
issn 1944-8244
year published 2017
volume 9
issue 2
beginning page 1331
ending page 1342
digital object identifier (doi) 10.1021/acsami.6b14407
web of science category Nanoscience & Nanotechnology; Materials Science, Multidisciplinary
subject category Science & Technology - Other Topics; Materials Science
unique article identifier WOS:000392458300019
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