Self-assembled RGD dehydropeptide hydrogels for drug delivery applications
authors Vilaca, H; Castro, T; Costa, FMG; Melle-Franco, M; Hilliou, L; Hamley, IW; Castanheira, EMS; Martins, JA; Ferreira, PMT
nationality International
journal JOURNAL OF MATERIALS CHEMISTRY B
keywords SUPRAMOLECULAR HYDROGELS; MOLECULAR SIMULATION; BUILDING-BLOCKS; ENERGY-TRANSFER; QUANTUM YIELDS; CELL-GROWTH; PEPTIDE; CURCUMIN; DESIGN; NANOSTRUCTURES
abstract Peptide-based self-assembled hydrogels have triggered remarkable research interest in recent years owing to their biocompatibility and biomimetic properties and responsiveness, which warrant many technological and biomedical applications. Dehydrodipeptides N-capped with naproxen emerged from our research as effective hydrogelators endowed with resistance to proteolysis. Dehydrodipeptide-based hydrogels are promising nanocarriers for drug delivery applications. In this work, we demonstrate that dehydrodipetide Npx-L-Ala-Z-DPhe-OH can be deployed as a minimalist hydrogelator module for synthesizing a gelating construct Npx-L-Ala-Z-DPhe-G-R-G-D-G-OH bearing a GRGDG adhesion motif. The self-assembly of the peptide construct and the drug delivery properties of the hydrogel were studied in this work. The peptide construct showed no toxicity towards a fibroblast cell line expressing the alpha(v)beta(3) integrin. Docking studies suggest that the hydrogelator block does not interfere with the recognition of the RGD motif by the integrin receptor. The self-assembly seems to be directed by intermolecular naphthalene p-p stacking interactions, with the peptide backbone assuming a random coil conformation both in solution and in the gel phase. TEM and STEM imaging revealed that the hydrogel is made of entangled bundles of long thin fibres (width circa 23 nm). The hydrogel exhibits viscoelastic properties, thermo-reversibility and recovery after mechanical fluidization. FRET studies showed that curcumin incorporated into the hydrogel interacts non-covalently with the hydrogel fibrils. Delivery of curcumin from the hydrogel into Nile red loaded model membranes (SUVs) was demonstrated by FRET. Naproxen N-capped dehydrodipeptides are efficacious minimalist hydrogelator modules for obtaining hydrogels functionalized with peptide ligands for cell receptors. These hydrogels are potential nanocarriers for drug delivery.
publisher ROYAL SOC CHEMISTRY
issn 2050-750X
year published 2017
volume 5
issue 43
beginning page 8607
ending page 8617
digital object identifier (doi) 10.1039/c7tb01883e
web of science category Materials Science, Biomaterials
subject category Materials Science
unique article identifier WOS:000414782200020
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journal analysis (jcr 2017):
journal impact factor 4.776
5 year journal impact factor 4.959
category normalized journal impact factor percentile 77.273
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