Differential effects of graphene oxide nanosheets on Candida albicans phagocytosis by murine peritoneal macrophages
authors Diez-Orejas, R; Feito, MJ; Cicuendez, M; Rojo, JM; Portoles, MT
nationality International
journal JOURNAL OF COLLOID AND INTERFACE SCIENCE
author keywords Graphene oxide; Macrophage; Candida albicans; Phagocytosis; Nanomaterial; Immune response
keywords ANTICANDIDA ANTIBODIES; CARBON NANOMATERIALS; VIRULENCE FACTORS; DRUG-DELIVERY; CANCER; RECOGNITION; INFECTION; PROTECTION; RESPONSES; IMMUNITY
abstract Macrophages, as effector cells involved in the innate and adaptive immunity, play a key role in the response to nanomaterials as graphene oxide (GO) and in their cellular uptake. The interactions at the interface of GO nanosheets, macrophages and microbial pathogens need to be assessed to determine the possible impairment of the immune system induced by biomedical treatments with this nanomaterial. Here, we have evaluated by flow cytometry and confocal microscopy the ability of murine peritoneal macrophages to phagocytose the fungal pathogen Candida albicans, alive or heat-killed, after treatment with poly(ethylene glycol-amine)-derivatized GO nanosheets (PEG-GO). After GO treatment, differences in fungal phagocytosis were observed between macrophages that had taken up GO nanosheets (GO* population) and those that had not (GO(-) population). GO treatment increased the ingested alive yeasts in GO(-) macrophages, whereas phagocytosis diminished in the GO' population. Ingestion of heat-killed yeasts was slightly higher in both GO(-) and GO(+) populations when comparing with control macrophages. For the first time, we show that GO uptake by macrophages modulates its phagocytic capability, affecting differentially the subsequent ingestion of either alive or heat-killed yeasts. Enhanced ingestion of heat killed yeast by GO-treated macrophages suggests a beneficial role of this nanomaterial for the clearance of dead microorganisms during infection. (C) 2017 Elsevier Inc. All rights reserved.
publisher ACADEMIC PRESS INC ELSEVIER SCIENCE
issn 0021-9797
year published 2018
volume 512
beginning page 665
ending page 673
digital object identifier (doi) 10.1016/j.jcis.2017.10.104
web of science category Chemistry, Physical
subject category Chemistry
unique article identifier WOS:000418729500073
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journal analysis (jcr 2019):
journal impact factor 7.489
5 year journal impact factor 6.171
category normalized journal impact factor percentile 80.818
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