NMR Metabolomics Reveals Metabolism-Mediated Protective Effects in Liver (HepG2) Cells Exposed to Subtoxic Levels of Silver Nanoparticles
authors Carrola, J; Pinto, RJB; Nasirpour, M; Freire, CSR; Gil, AM; Santos, C; Oliveira, H; Duarte, IF
nationality International
journal JOURNAL OF PROTEOME RESEARCH
author keywords liver (HepG2) cells; silver nanoparticles (AgNPs); ionic silver; NMR metabolomics; cell metabolism
keywords CYTOTOXICITY; AUTOPHAGY; NANOTOXICOLOGY; INHIBITION; TOXICITY; HEPATOMA; DEATH
abstract The expansion of biomedical and therapeutic applications of silver nanoparticles (AgNPs) raises the need to further understand their biological effects on human cells. In this work, NMR metabolomics has been applied to reveal the metabolic effects of AgNPs toward human hepatoma (HepG2) cells, which are relevant with respect to nanoparticle accumulation and detoxification. Cellular responses to widely disseminated citrate-coated AgNPs (Cit30) and to emergent biogenic AgNPs prepared using an aqueous plant extract as reducing and stabilizing agent (GS30) have been compared with a view to assess the influence of nanoparticle I coating on the metabolic effects produced. Subtoxic concentrations (IC5 and IC20) of both nanoparticle types caused profound changes in the cellular metabolome, suggesting adaptations in energy production processes (glucose metabolism and the phosphocreatine system), antioxidant defenses, protein degradation and lipid metabolism. These signatures were proposed to reflect mainly metabolism-mediated protective mechanisms and were found to be largely common to Cit30 and GS30 AgNPs, although differences in the magnitude of response, not captured by conventional cytotoxicity assessment, were detected. Overall, this study highlights the value of NMR metabolomics for revealing subtoxic biological effects and helping to understand cell-nanomaterial interactions.
publisher AMER CHEMICAL SOC
issn 1535-3893
year published 2018
volume 17
issue 4
beginning page 1636
ending page 1646
digital object identifier (doi) 10.1021/acs.jproteome.7b00905
web of science category Biochemical Research Methods
subject category Biochemistry & Molecular Biology
unique article identifier WOS:000429886600027
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