An integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible aqueous biphasic systems
authors Ferreira, AM; Passos, H; Okafuji, A; Tavares, APM; Ohno, H; Freire, MG; Coutinho, JAP
nationality International
journal GREEN CHEMISTRY
keywords AMMONIUM-BASED ZWITTERIONS; IONIC LIQUIDS; POLYETHYLENE-GLYCOL; 2-PHASE SYSTEMS; ORGANIC-SOLVENTS; PHASE; EXTRACTION; PROTEINS; BEHAVIOR; LACCASE
abstract Herein, thermoreversible aqueous biphasic systems (ABS) composed of ammonium-based zwitterions (ZIs) and polymers are disclosed to act as integrated bioreaction-separation processes. The biocatalytic reaction involving laccase occurs in homogeneous media, after which small changes in temperature induce the formation of two phases and the complete separation of the enzyme from the products in a single-step. These systems also allow the recovery and reuse of the enzyme, along with the ZI-rich phase, contributing towards the development of sustainable biocatalytic processes.
publisher ROYAL SOC CHEMISTRY
issn 1463-9262
year published 2018
volume 20
issue 6
beginning page 1218
ending page 1223
digital object identifier (doi) 10.1039/c7gc03880a
web of science category Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY
subject category Chemistry; Science & Technology - Other Topics
unique article identifier WOS:000428088400007
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journal impact factor 8.586
5 year journal impact factor 8.717
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