Adhesive free-standing multilayer films containing sulfated levan for biomedical applications
authors Gomes, TD; Caridade, SG; Sousa, MP; Azevedo, S; Kandur, MY; Oner, ET; Alves, NM; Mano, JF
nationality International
journal ACTA BIOMATERIALIA
author keywords Sulfated levan; Free-standing membranes; Adhesiveness; Biomedical applications
keywords GENIPIN CROSS-LINKING; POLYSACCHARIDE NANOSHEET; VISCOELASTIC PROPERTIES; CHITOSAN MEMBRANES; CELLULAR ADHESION; HYALURONIC-ACID; ALGINATE; CELLS; MICROENVIRONMENTS; DIFFERENTIATION
abstract This work is the first reporting the use of layer-by-layer to produce adhesive free-standing (FS) films fully produced using natural-based macromolecules: chitosan (CHI), alginate (ALG) and sulfated levan (L-S). The deposition conditions of the natural polymers were studied through zeta potential measurements and quartz crystal microbalance with dissipation monitoring analysis. The properties of the FS films were evaluated and compared with the control ones composed of only CHI and ALG in order to assess the influence of levan polysaccharide introduced in the multilayers. Tensile tests, dynamic mechanical analysis and single lap shear strength tests were performed to evaluate the mechanical properties of the prepared FS films. The presence of L-S conferred both higher tensile strength and shear strength to the developed FS membranes. The results showed an adhesion strength 4 times higher than the control (CHI/ALG) FS films demonstrating the adhesive character of the FS films containing L-S. Morphological and topography studies were carried out revealing that the crosslinking reaction granted the L-S based FS film with a higher roughness and surface homogeneity. Preliminary biological assays were performed by cultivating myoblasts cells on the surface of the produced FS films. Both crosslinked and uncrosslinked FS films containing L-S were cytocompatible and myoconductive. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
publisher ELSEVIER SCI LTD
issn 1742-7061
year published 2018
volume 69
beginning page 183
ending page 195
digital object identifier (doi) 10.1016/j.actbio.2018.01.027
web of science category Engineering, Biomedical; Materials Science, Biomaterials
subject category Engineering; Materials Science
unique article identifier WOS:000427334100014
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journal analysis (jcr 2019):
journal impact factor 7.242
5 year journal impact factor 7.502
category normalized journal impact factor percentile 89.769
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