Comparative proteomic analyses of urine from rat urothelial carcinoma chemically induced by exposure to N-butyl-N-(4-hydroxybutyl)-nitrosamine

authors	Ferreira, R; Oliveira, P; Martins, T; Magalhaes, S; Trindade, F; Pires, MJ; Colaco, B; Barros, A; Santos, L; Amado, F; Vitorino, R
nationality	International
journal	MOLECULAR BIOSYSTEMS
keywords	BLADDER-CANCER BIOMARKERS; TYPE-1 DIABETES-MELLITUS; DISCOVERY; CARCINOGENESIS; PROTEIN; EXPRESSION; PEPTIDES; KIDNEY; TUMORS; DNA
abstract	Bladder cancer is estimated to be the ninth most common malignancy with a high rate of recurrence and progression despite therapy, early diagnosis being crucial for timely intervention. Using a well-established animal model of urothelial carcinoma, we performed a comprehensive analysis of urine proteome profile from healthy animals and animals with urothelial carcinoma at two time-points of disease pathogenesis. GeLC-MS/MS, followed by bioinformatics analysis of unique proteins and the ones present in significantly distinct levels among groups, highlighted the biological processes involved in disease pathogenesis such as, for instance, response to selenium and to drugs, neutral lipid metabolism at earlier stages of disease, and inflammation, immune response and wound healing at advanced stages. Proteins from up-regulated biological processes might be seen as putative disease biomarkers. These include, for example, cadherins, lipoproteins, and glysosyltransferases, which may be included in multimarker strategies. Taken together, the data support the application of urine proteomics for the identification of the biological processes modulated by bladder cancer in an integrative perspective. The present exploratory urinary proteomic analysis might be seen as an important starting point for studies targeting urinary proteins in human, aiming at the implementation of novel laboratory approaches for the detection and successful management of urothelial carcinoma.
publisher	ROYAL SOC CHEMISTRY
issn	1742-206X
year published	2015
volume	11
issue	6
beginning page	1594
ending page	1602
digital object identifier (doi)	10.1039/c4mb00606b
web of science category	Biochemistry & Molecular Biology
subject category	Biochemistry & Molecular Biology
unique article identifier	WOS:000354792500013