Hybrids Based on Graphene Oxide and Porphyrin as Tools for Detection and Stabilization of DNA G-Quadruplexes

authors	Monteiro, AR; Ramos, CIV; Fateixa, S; Moura, NMM; Neves, MGPMS; Trindade, T
nationality	International
journal	ACS OMEGA
keywords	CATIONIC PORPHYRIN; PHOTOTHERMAL THERAPY; BRAIN CANCER; LIGANDS; FUNCTIONALIZATION; RECOGNITION; DERIVATIVES; TELOMERASE; SURFACE; RAMAN
abstract	Telomerase inhibition has been an important strategy in cancer therapies, but for which effective drugs are still required. Here, noncovalent hybrid nanoplatforms containing the tetracationic 5,10,15,20-tetrakis(1-methyl-pyridinium-4-yl) porphyrin (TMPyP) and graphene oxide (GO) were prepared for promoting telomerase inhibition through the selective detection and stabilization of DNA guanine-quadruplex (G-Q) structures. Upon binding TMPyP to the GO sheets, the typical absorption bands of porphyrin have been red-shifted and the fluorescence emission was quenched. Raman mapping was used for the first time to provide new insights into the role of the electrostatic and pi-pi stacking interactions in the formation of such hybrids. The selective recovery of fluorescence observed during the titration of TMPyP@GO with G-Q, resembles a selective "turn-off-on" fluorescence sensor for the detection of G-Q, paving the way for a new class of antitumor drugs.
publisher	AMER CHEMICAL SOC
issn	2470-1343
year published	2018
volume	3
issue	9
beginning page	11184
ending page	11191
digital object identifier (doi)	10.1021/acsomega.8b01366
web of science category	Chemistry, Multidisciplinary
subject category	Chemistry
unique article identifier	WOS:000446186000082