Prediction of protein partition in polymer/salt aqueous two-phase systems using the modified Wilson model
authors Madeira, PP; Xu, X; Teixeira, JA; Macedo, EA
nationality International
journal BIOCHEMICAL ENGINEERING JOURNAL
author keywords protein recovery; aqueous two-phase systems; modelling; excess gibbs energy; modified Wilson model; activity coefficients
keywords LOCAL COMPOSITION MODEL; EXCESS GIBBS ENERGY; THERMODYNAMIC PROPERTIES; POLY(ETHYLENE GLYCOL); MOLECULAR-WEIGHT; ELECTROLYTE SYSTEMS; LIQUID-EQUILIBRIUM; BIPHASIC SYSTEMS; PHASE SYSTEMS; EQUATION
abstract The extension of the modified Wilson model to multicomponent mixtures, presented in a previous publication, is applied to predict the partition of the following proteins: bovine serum albumin (BSA), lysozyme, glucosidase and catalase, in the Na2SO4/PEG6000 and K2HPO4/PEG6000 aqueous two-phase systems at 298.15 K. The results obtained with the model are, in general, in fair agreement with the experimental data. In the modelling methodology adopted here, special emphasis on the so-called "charge effects" to the protein partition was given. To our knowledge, no experimental information is available in the literature that allows to estimate the interaction parameters between these macromolecules and the components present in the aqueous two-phase systems (water, salts and polymer). Thus, the deviations observed between calculated and experimental protein partition are mainly due to some assumptions made in the predictive methodology. (c) 2005 Elsevier B.V. All rights reserved.
publisher ELSEVIER SCIENCE SA
issn 1369-703X
year published 2005
volume 24
issue 2
beginning page 147
ending page 155
digital object identifier (doi) 10.1016/j.bej.2005.02.004
web of science category Biotechnology & Applied Microbiology; Engineering, Chemical
subject category Biotechnology & Applied Microbiology; Engineering
unique article identifier WOS:000229352600007
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