"On the Collander equation": Protein partitioning in polymer/polymer aqueous two-phase systems
authors Madeira, PP; Teixeira, JA; Macedo, EA; Mikheeva, LM; Zaslavsky, BY
nationality International
journal JOURNAL OF CHROMATOGRAPHY A
author keywords aqueous two-phase systems; protein partitioning; collander equation
keywords SOLVATION ENERGY RELATIONSHIP; BIPHASIC SYSTEMS; POLYETHYLENE-GLYCOL; ORGANIC-MOLECULES; METHYLENE GROUP; HYDROPHOBICITY; ACID; CHARACTER; PHASES; SALT
abstract Distribution coefficients of randomly selected proteins were measured in aqueous two-phase systems (ATPSs) formed by different combinations of Dextran-75 (Dex), Ficoll-70, polyethylene glycol-8000 (PEG), hydroxypropyl starch-100 (PES), and Ucon50HB5100 (Ucon, a random copolymer of ethylene glycol and propylene glycol) at particular polymer concentrations, all containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4. Most of the proteins in the PEG-Ucon system precipitated at the interface. In the other ATPSs, namely, PES-PEG, PES-Ucon, Ficoll-PEG, Ficoll-Ucon, and in Dex-PEG and Dex-Ucon described earlier the distribution coefficients for the proteins were correlated according to the solvent regression equation: In K-i = a(io) In K-o + b(io) where K-i and K-o are the distribution coefficients for any protein in the ith and oth two-phase systems. Coefficients a(io) and b(io) are constants, the values of which depend upon the particular compositions of the two-phase systems under comparison. (C) 2008 Published by Elsevier B.V.
publisher ELSEVIER SCIENCE BV
issn 0021-9673
year published 2008
volume 1190
issue 1-2
beginning page 39
ending page 43
digital object identifier (doi) 10.1016/j.chroma.2008.03.003
web of science category Biochemical Research Methods; Chemistry, Analytical
subject category Biochemistry & Molecular Biology; Chemistry
unique article identifier WOS:000255848600006
  ciceco authors
  impact metrics
journal analysis (jcr 2019):
journal impact factor 4.049
5 year journal impact factor 3.861
category normalized journal impact factor percentile 84.034
dimensions (citation analysis):
altmetrics (social interaction):



 


Sponsors

1suponsers_list_ciceco.jpg