Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems
authors Madeira, PP; Teixeirab, JA; Macedo, EA; Mikheeva, LM; Zaslavsky, BY
nationality International
journal FLUID PHASE EQUILIBRIA
author keywords aqueous two-phase systems; protein partitioning; Collander equation
keywords SOLVATION ENERGY RELATIONSHIP; BIPHASIC SYSTEMS; POLYETHYLENE-GLYCOL; PARTITION-COEFFICIENTS; ORGANIC-MOLECULES; METHYLENE GROUP; PEPTIDES; PROTEINS; DEXTRAN; POLYMER
abstract Distribution coefficients for a variety of proteins and certain other biomolecules (peptides, amino acids, and carbohydrates) (overall 27 different solutes) were measured in aqueous two-phase systems (ATPSs) dextran (Dex)-polyethylene glycol (PEG) and Dex-Ucon 50-HB-5100 (Ucon-a random copolymer of ethylene glycol and propylene glycol) both containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4, at 23 degrees C. Distribution coefficients of some selected solutes were also measured in the above two-phase systems at three different polymer concentrations for each system. It was established that the distribution coefficients for all the proteins examined in the ATPSs are correlated according to the so-called Collander linear equation. (C) 2008 Elsevier B.V. All rights reserved.
publisher ELSEVIER SCIENCE BV
issn 0378-3812
year published 2008
volume 267
issue 2
beginning page 150
ending page 157
digital object identifier (doi) 10.1016/j.fluid.2008.03.003
web of science category Thermodynamics; Chemistry, Physical; Engineering, Chemical
subject category Thermodynamics; Chemistry; Engineering
unique article identifier WOS:000256174400008
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