Analyzing the effects of protecting osmolytes on solute-water interactions by solvatochromic comparison method: II. Globular proteins
authors Ferreira, LA; Fan, X; Madeira, PP; Kurgan, L; Uversky, VN; Zaslavsky, BY
nationality International
journal RSC ADVANCES
keywords AQUEOUS 2-PHASE SYSTEMS; TRIMETHYLAMINE-N-OXIDE; PREFERENTIAL INTERACTIONS; SOLVENT INTERACTIONS; ELASMOBRANCH FISHES; UNFOLDED PROTEINS; AMINO-ACIDS; STABILIZATION; PREDICTION; MECHANISM
abstract Partitioning of 11 globular proteins was examined in aqueous dextran-PEG-sodium/potassium phosphate buffer (0.01 M K/NaPB, pH 7.4) two-phase systems (ATPSs) containing 0.5 M sorbitol. The data obtained were analyzed together with those reported previously for the same proteins in osmolyte-free ATPS and ATPS containing 0.5 M sucrose, TMAO, or trehalose. It was found that all the partition coefficients for proteins determined in the presence of 0.5 M of different osmolytes and in the absence of osmolytes may be described in terms of the differences between solvent properties of the coexisting phases. Solute-specific coefficients characterizing different types of solute-solvent interactions were calculated for each protein. These solute-specific coefficients are linearly interrelated implying cooperativity of different types of protein-water interactions. The data obtained indicate the lack of any association of the aforementioned osmolytes at concentration of 0.5 M with proteins. Computational analysis of one of the solute-specific coefficient Ss-values characterizing dipole-dipole protein-water interactions shows that it is determined by the peculiarities of protein surface.
publisher ROYAL SOC CHEMISTRY
issn 2046-2069
year published 2015
volume 5
issue 73
beginning page 59780
ending page 59791
digital object identifier (doi) 10.1039/c5ra08612d
web of science category Chemistry, Multidisciplinary
subject category Chemistry
unique article identifier WOS:000357961800098
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journal impact factor 3.119
5 year journal impact factor 3.098
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