Analyzing the effects of protecting osmolytes on solute-water interactions by solvatochromic comparison method: I. Small organic compounds
authors Ferreira, LA; Madeira, PP; Uversky, VN; Zaslavsky, BY
nationality International
journal RSC ADVANCES
keywords AQUEOUS 2-PHASE SYSTEMS; PROTEIN-SOLVENT INTERACTIONS; TRIMETHYLAMINE-N-OXIDE; AMINO-ACIDS; SALT ADDITIVES; PARTITION; COEFFICIENTS; SCALE; SPECTROSCOPY; SOLUBILITIES
abstract Solvent properties of water (dipolarity/polarizability, hydrogen bond donor (HBD) acidity, and hydrogen bond acceptor (HBA) basicity) in aqueous solutions of osmolytes (sorbitol, sucrose, trehalose, and trimethylamine N-oxide (TMAO)) were studied at different osmolyte concentrations using a solvatochromic comparison method. The solvent properties of aqueous media in the coexisting phases of aqueous dextran-PEG-sodium/potassium phosphate buffer (0.01 M K/NaPB, pH 7.4) two-phase system (ATPS) containing 0.5 M osmolyte additive (sorbitol, sucrose, trehalose, TMAO) and osmolyte-free ATPS were characterized. Partitioning of 30 low molecular weight polar organic compounds was examined in aqueous dextran-PEG-0.01 M K/NaPB ATPS containing 0.5 M sorbitol. The solute-specific coefficients for the compounds examined were determined from the data obtained here and those reported previously. The results obtained demonstrate that the osmolytes examined affect the partition behavior of organic compounds in ATPS by influencing solvent properties of the media and not by direct association with the compounds.
publisher ROYAL SOC CHEMISTRY
issn 2046-2069
year published 2015
volume 5
issue 74
beginning page 59812
ending page 59822
digital object identifier (doi) 10.1039/c5ra08610h
web of science category Chemistry, Multidisciplinary
subject category Chemistry
unique article identifier WOS:000358230400001
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journal impact factor 3.119
5 year journal impact factor 3.098
category normalized journal impact factor percentile 59.04
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