Analyzing the effects of protecting osmolytes on solute-water interactions by solvatochromic comparison method: I. Small organic compounds

authors	Ferreira, LA; Madeira, PP; Uversky, VN; Zaslavsky, BY
nationality	International
journal	RSC ADVANCES
keywords	AQUEOUS 2-PHASE SYSTEMS; PROTEIN-SOLVENT INTERACTIONS; TRIMETHYLAMINE-N-OXIDE; AMINO-ACIDS; SALT ADDITIVES; PARTITION; COEFFICIENTS; SCALE; SPECTROSCOPY; SOLUBILITIES
abstract	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor (HBD) acidity, and hydrogen bond acceptor (HBA) basicity) in aqueous solutions of osmolytes (sorbitol, sucrose, trehalose, and trimethylamine N-oxide (TMAO)) were studied at different osmolyte concentrations using a solvatochromic comparison method. The solvent properties of aqueous media in the coexisting phases of aqueous dextran-PEG-sodium/potassium phosphate buffer (0.01 M K/NaPB, pH 7.4) two-phase system (ATPS) containing 0.5 M osmolyte additive (sorbitol, sucrose, trehalose, TMAO) and osmolyte-free ATPS were characterized. Partitioning of 30 low molecular weight polar organic compounds was examined in aqueous dextran-PEG-0.01 M K/NaPB ATPS containing 0.5 M sorbitol. The solute-specific coefficients for the compounds examined were determined from the data obtained here and those reported previously. The results obtained demonstrate that the osmolytes examined affect the partition behavior of organic compounds in ATPS by influencing solvent properties of the media and not by direct association with the compounds.
publisher	ROYAL SOC CHEMISTRY
issn	2046-2069
year published	2015
volume	5
issue	74
beginning page	59812
ending page	59822
digital object identifier (doi)	10.1039/c5ra08610h
web of science category	Chemistry, Multidisciplinary
subject category	Chemistry
unique article identifier	WOS:000358230400001