|
authors |
da Silva, NR; Ferreira, LA; Madeira, PP; Teixeira, JA; Uversky, VN; Zaslavsky, BY |
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nationality |
International |
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journal |
JOURNAL OF CHROMATOGRAPHY A |
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author keywords |
Aqueous two-phase system; Partitioning; Proteins; Solute-water interactions; Solvatochromic comparison method; Solvent properties |
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keywords |
PROTEINS; OPTIMIZATION; EXTRACTION; PURIFICATION; ANTIBODIES; MECHANISMS; PARTITION; TRYPSIN |
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abstract |
Partition behavior of eight small organic compounds and six proteins was examined in poly(ethylene glycol)-8000-sodium sulfate aqueous two-phase systems containing 0.215 M NaCl and 0.5 M osmolyte (sorbitol, sucrose, TMAO) and poly(ethylene glycol)-10000-sodium sulfate-0.215 M NaCl system, all in 0.01 M sodium phosphate buffer, pH 6.8. The differences between the solvent properties of the coexisting phases (solvent dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) were characterized with solvatochromic dyes using the solvatochromic comparison method. Differences between the electrostatic properties of the phases were determined by analysis of partitioning of sodium salts of dinitrophenylated (DNP-) amino acids with aliphatic alkyl side-chain. The partition coefficients of all compounds examined (including proteins) were described in terms of solute-solvent interactions. The results obtained in the study show that solute-solvent interactions of nonionic organic compounds and proteins in polyethylene glycol-sodium sulfate aqueous two-phase system change in the presence of NaCl additive. (C) 2015 Elsevier B.V. All rights reserved. |
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publisher |
ELSEVIER SCIENCE BV |
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issn |
0021-9673 |
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year published |
2015 |
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volume |
1425 |
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beginning page |
51 |
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ending page |
61 |
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digital object identifier (doi) |
10.1016/j.chroma.2015.11.019 |
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web of science category |
Biochemical Research Methods; Chemistry, Analytical |
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subject category |
Biochemistry & Molecular Biology; Chemistry |
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unique article identifier |
WOS:000366770400007
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