Interrelationship between partition behavior of organic compounds and proteins in aqueous dextran-polyethylene glycol and polyethylene glycol-sodium sulfate two-phase systems
authors Ferreira, LA; da Silva, NR; Wlodarczyk, SR; Loureiro, JA; Madeira, PP; Teixeira, JA; Uversky, VN; Zaslaysky, BY
nationality International
journal JOURNAL OF CHROMATOGRAPHY A
author keywords Aqueous two-phase partitioning; Aqueous two phase systems; Dex-PEG system; PEG-sodium sulfate system; Mononucleotides; Phosphate group
keywords SOLUTE-SOLVENT INTERACTIONS; SALT ADDITIVES
abstract Partition behavior of adenosine and guanine mononucleotides was examined in aqueous dextran-polyethylene glycol (PEG) and PEG-sodium sulfate two-phase systems. The partition coefficients for each series of mononucleotides were analyzed as a functions of the number of phosphate groups and found to be dependent on the nature of nucleic base and on the type of ATPS utilized. It was concluded that an average contribution of a phosphate group into logarithm of partition coefficient of a mononucleotide cannot be used to estimate the difference between the electrostatic properties of the coexisting phases of ATPS. The data obtained in this study were considered together with those for other organic compounds and proteins reported previously, and the linear interrelationship between logarithms of partition coefficients in dextran-PEG, PEG-Na2SO4 and PEG-Na2SO4-0.215 M NaCl (all in 0.01 M Na- or K/Na-phosphate buffer, pH 7.4 or 6.8) was established. Similar relationship was found for the previously reported data for proteins in Dex-PEG, PEG-600-Na2SO4, and PEG-8000-Na2SO4 ATPS. It is suggested that the linear relationships of the kind established in ATPS may be observed for biological properties of compounds as well. (C) 2016 Elsevier B.V. All rights reserved.
publisher ELSEVIER SCIENCE BV
issn 0021-9673
year published 2016
volume 1443
beginning page 21
ending page 25
digital object identifier (doi) 10.1016/j.chroma.2016.03.032
web of science category Biochemical Research Methods; Chemistry, Analytical
subject category Biochemistry & Molecular Biology; Chemistry
unique article identifier WOS:000374362600003
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