Improved model systems for bacterial membranes from differing species: The importance of varying composition in PE/PG/cardiolipin ternary mixtures
authors Lopes, SC; Neves, CS; Eaton, P; Gameiro, P
nationality International
journal MOLECULAR MEMBRANE BIOLOGY
author keywords Antimicrobial; bacterial membranes; cardiolipin; dynamic light scattering; fluorescence anisotropy; atomic force microscopy
keywords ATOMIC-FORCE MICROSCOPY; CATIONIC ANTIMICROBIAL PEPTIDES; THERMOTROPIC PHASE-BEHAVIOR; ACYL-CHAIN LENGTH; ESCHERICHIA-COLI; FLUORESCENT-PROBES; LIGHT-SCATTERING; PLANAR BILAYERS; X-RAY; CARDIOLIPIN
abstract Steady-state fluorescence anisotropy and dynamic light scattering (DLS) were used to determine the thermotropic properties of lipid systems that act as models for bacterial membranes of Yersinia kristensenii and Proteus mirabilis. Lipid proportions of PE: PG: CL of 0.60:0.20:0.20 and 0.80:0.15:0.05, were used in order to mimic these two membranes respectively. We observed that the introduction of cardiolipin (CL) as a third lipid component of any PE: PG mixture, changes the system's properties considerably. The results obtained by these two techniques show that the main transition temperatures obtained are undoubtedly CL-dependent. Additionally AFM experiments were performed and these results show that even at small concentration CL produces important changes not only in the membrane thermotropic properties, but also in the bilayer structure. In summary, we were able to compare how low and high CL concentration affect bacterial membrane model system properties which can provide a further explanation for the different antibiotic susceptibilities reported for Y. kristensenii and P. mirabilis.
publisher INFORMA HEALTHCARE
issn 0968-7688
year published 2012
volume 29
issue 6
beginning page 207
ending page 217
digital object identifier (doi) 10.3109/09687688.2012.700491
web of science category Biochemistry & Molecular Biology; Cell Biology
subject category Biochemistry & Molecular Biology; Cell Biology
unique article identifier WOS:000309471800002
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journal analysis (jcr 2019):
journal impact factor 2.5
5 year journal impact factor 2.475
category normalized journal impact factor percentile 31.142
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