R-phycoerythrin extraction and purification from fresh Gracilaria sp. using thermo-responsive systems
authors Vicente, FA; Cardoso, IS; Martins, M; Goncalves, CVM; Dias, ACRV; Domingues, P; Coutinho, JAP; Ventura, SPM
nationality International
journal GREEN CHEMISTRY
keywords GRATELOUPIA-TURUTURU; 2-PHASE SYSTEMS; IONIC LIQUIDS; CYTOCHROME-C; PROTEINS; SURFACTANTS; SEPARATION
abstract R-phycoerythrin is a high added-value protein found in red macroalgae with several interesting properties. Despite the promising results found when R-phycoerythrin is used as an optically active center in luminescent solar concentrators (LSCs), it still has some problems that can be attributed to the low stability of the R-phycoerythrin in the presence of the specific contaminant proteins found in the crude extract. The development of downstream strategies able to reduce the use of environmentally hazardous solvents, while improving the purification without compromising the R-phycoerythrin structural integrity is still the biggest challenge to overcome. Aqueous micellar two-phase systems (AMTPS) appear as an appealing fractionation approach since they allow the processing of systems with larger water contents, while displaying great selectivity and biocompatibility with several biomolecules. Moroever, AMTPS that mix surfactants and surface-active ionic liquids are shown to significantly enhance protein purification. In this work, mixed AMTPS were applied to the R-phycoerythrin purification from red macroalgae. After the process optimization, this work proposes the application of two consecutive steps of purification as the final process to isolate R-phycoerythrin from the remaining proteins composing the crude extract, while maintaining the structural integrity of R-phycoerythrin, as requested to be used in the LSC. Besides a good performance, the two-step approach developed was also shown to have a lower environmental impact with a carbon footprint decrease of 16%, when compared with the conventional AMTPS.
publisher ROYAL SOC CHEMISTRY
issn 1463-9262
isbn 1463-9270
year published 2019
volume 21
issue 14
beginning page 3816
ending page 3826
digital object identifier (doi) 10.1039/c9gc00104b
web of science category Chemistry, Multidisciplinary; Green & Sustainable Science & Technology
subject category Chemistry; Science & Technology - Other Topics
unique article identifier WOS:000475506200024

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