MoS2 flakes stabilized with DNA/RNA nucleotides: In vitro cell response

authors	Cicuendez, M; Silva, VS; Santos, J; Coimbra, A; Oliveira, H; Ayan-Varela, M; Paredes, JI; Villar-Rodil, S; Vila, M
nationality	International
journal	MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
author keywords	Transition metal dichalcogenides (TMDCs); MoS2; Biodispersants; Biocompatibility; in vitro cell response; Osteoblast
keywords	MOLYBDENUM-DISULFIDE; AQUEOUS DISPERSIONS; GRAPHENE ANALOGS; DRUG-DELIVERY; NANOSHEETS; EXFOLIATION; DICHALCOGENIDES; NANOPARTICLES; NANOMATERIALS; CULTURE
abstract	Two-dimensional transition metal dichalcogenides (TMDCs), such as MoS2 and WS2, have recently emerged as nanomaterials with potential use in biomedicine. An attractive means to favor their interaction with biological media is the use of proper biomolecules as exfoliating/dispersing agents. Here, MoS2 flakes were stabilized with different small functional biomolecules such as adenosine monophosphate (AMP), guanosine monophosphate (GMP) and flavin mononucleotide (FMN) through the strong nucleotide - MoS2 interaction of Lewis acid-base type, rather than just on the weak dispersive and hydrophobic forces commonly associated with the use of many surfactants. The impact of the nucleotide-stabilized MoS2 flakes on the viability and cell proliferation, on the production of intracellular reactive oxygen species (ROS), and on the preosteoblast differentiation process (early stage) has been also evaluated, as well as the incorporation and intracellular localization of the nanomaterials by MC3T3-E1 and Saos-2 cells. The nucleotide-stabilized MoS2 flakes were found to exhibit excellent biocompatibility. Furthermore, their incorporation did not affect the integrity of the cell plasma membrane, which makes them ideal candidates for delivering drug/gene directly into cells. The in vitro cell response of tumor cells to these nanomaterials differs from that of undifferentiated cells, which provides the basis for their potential use in cancer therapy.
publisher	ELSEVIER SCIENCE BV
issn	0928-4931
isbn	1873-0191
year published	2019
volume	100
beginning page	11
ending page	22
digital object identifier (doi)	10.1016/j.msec.2019.02.002
web of science category	Materials Science, Biomaterials
subject category	Materials Science
unique article identifier	WOS:000466059700002