Effect of osmolytes on the activity of anti-cancer enzyme L-Asparaginase II from Erwinia chrysanthemi
authors Wlodarczyk, SR; Costa-Silva, TA; Pessoa, A; Madeira, P; Monteiro, G
nationality International
journal PROCESS BIOCHEMISTRY
author keywords Protein stability; L-asparaginase; Enzyme formulation; Biopharmaceutical; Protein aggregates; Protein-based pharmaceuticals
keywords PROTEIN STABILITY; STABILIZATION; EXPRESSION; MECHANISM; PROTECTION; ARGININE; SUCROSE
abstract L-asparaginase is used for the treatment of acute lymphoblastic leukaemia (ALL); however, its formulation presents drawbacks such as a lack of stability and formation of aggregates. Osmolytes are small molecules accumulated by cells in response to environmental stresses and present a protective behaviour, favouring the equilibrium of macromolecules towards the native conformation. Therefore, osmolytes are employed as excipients in pharmaceutical protein formulations. Herein, recombinant L-ASNase II from Erwinia chrysanthemi (ErA II) was analysed with respect to the effect of osmolytes on kinetic and stability of this biopharmaceutical, The aggregation profiles were analysed trough nanotracking particle analysis and dynamic light scattering. The majority of the tested osmolytes increased ErA II specific activity and stability, being more pronounced for sucrose and sorbitol, which increased almost 70% of ErA II activity. The polyol preserved total enzyme activity for 30 days while sucrose preserved 81.1 +/- 5.3% total enzyme activity over this period. Each osmolyte resulted in a specific aggregation profile and the presence of sucrose or sorbitol resulted in a lower quantity of aggregates in the range of 100-300 nm. The present findings may contribute to the improvement of adjuvants in L-ASNase formulations and the optimization of other biopharmaceutical formulations.
publisher ELSEVIER SCI LTD
issn 1359-5113
isbn 1873-3298
year published 2019
volume 81
beginning page 123
ending page 131
digital object identifier (doi) 10.1016/j.procbio.2019.03.009
web of science category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical
subject category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering
unique article identifier WOS:000469158700017
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