Host-defense peptides AC12, DK16 and RC11 with immunomodulatory activity isolated from Hypsiboas raniceps skin secretion
authors Popov, CSFC; Magalhaes, BS; Goodfellow, BJ; Bocca, AL; Pereira, DM; Andrade, PB; Valentao, P; Pereira, PJB; Rodrigues, JE; Veloso, PHD; Rezende, TMB
nationality International
journal PEPTIDES
author keywords Peptide; Anura; Immunomodulatory; Anti-inflammatory; NMR
keywords TUMOR-NECROSIS-FACTOR; FROG-SKIN; ANTIMICROBIAL PEPTIDES; FACTOR-ALPHA; HUMAN MONOCYTES; IL-10; ANTICANCER; INFLAMMATION; EXPRESSION; CYTOKINES
abstract Inflammation is a natural defense mechanism of the immune system; however, when unregulated, it can lead to chronic illness. Glucocorticoids are the most commonly used agents to effectively treat inflammatory conditions, including autoimmune diseases, however these substances can trigger a number of side effects. Thus, viable alternatives to the use of these drugs would be advantageous. In this study, we have analyzed the anti-inflammatory profile of three synthetic peptides first identified in skin secretion of the tree frog Hypsiboas raniceps. Structural characterization was performed using NMR spectroscopy and Mass Spectrometry, and the peptides were tested in vitro in RAW 264.7 cells and in vivo in Balb/c mice for their functional properties. The samples did not show a significant antimicrobial profile. NMR spectroscopy indicated that AC12 (ACFLTRLGTYVC) has a disulfide bond between C2 and C11 and a beta-sheet-turn-beta-sheet conformation in aqueous solution. This peptide showed no cytotoxic effect in mammalian cells and it was the most effective in reducing anti-inflammatory markers, such as NO, TNF-alpha and IL-12. Peptide DK16 (DKERPICSNTFRGRKC) demonstrated anti-inflammatory properties in vitro, while RC11 (RCFRRRGKLTC) significantly altered the cell viability in RAW 264.7 but was shown to be safe in Balb/c erythrocytes. Our results indicate that, of the three peptides studied, AC12 is the most efficient in reducing anti-inflammatory markers, and it could be a potential agent for the treatment of inflammatory diseases.
publisher ELSEVIER SCIENCE INC
issn 0196-9781
year published 2019
volume 113
beginning page 11
ending page 21
digital object identifier (doi) 10.1016/j.peptides.2018.12.007
web of science category Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy
subject category Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy
unique article identifier WOS:000459508600002
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journal impact factor 2.851
5 year journal impact factor 2.761
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