Chromatographic Separation of Phenolic Compounds from Extra Virgin Olive Oil: Development and Validation of a New Method Based on a Biphenyl HPLC Column
authors Ferro, MD; Santos, SAO; Silvestre, AJD; Duarte, MF
nationality International
journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
author keywords extra virgin olive oil; hydrophilic phenolic compounds; chromatographic resolution; Kinetex biphenyl core-shell column; method validation
keywords REVERSED-PHASE HPLC; POLYPHENOLS; OLEUROPEIN; 3,4-DIHYDROXYPHENYLGLYCOL; ANTIOXIDANTS; PERFORMANCE; HYDROLYSIS; TYROSOL
abstract Three different high performance liquid chromatography columns were accessed for phenolic compounds (PC) separation in the hydrophilic fraction of extra virgin olive oil (EVOO). Two fully porous C-18 bonded silica phases and one partially porous biphenyl column were used. Biphenyl column allowed for an increase of more than 30% in peak capacity (n(c)), higher selectivity () (1.045), and improved retention (k), with a reduction of 22.1% in the retention time. The higher resolution (R-s) was obtained by using the biphenyl column, with a fair separation of oleuropein aglycone isomers (OAI) and a good identification of caffeic acid (CA). Tyrosol (T), hydroxytyrosol (HT), and dihydroxyphenyl glycol (DHPG) were also well separated and identified. Moreover, the method using a biphenyl column was fully validated according to the requirements for new methods. For all parameters, the method applying the biphenyl column proved to be a reliable, accurate, and robust tool for separation, identification, and quantification of the main PCs in EVOOs.
publisher MDPI
isbn 1422-0067
year published 2019
volume 20
issue 1
digital object identifier (doi) 10.3390/ijms20010201
web of science category Biochemistry & Molecular Biology; Chemistry, Multidisciplinary
subject category Biochemistry & Molecular Biology; Chemistry
unique article identifier WOS:000459747700201
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journal impact factor 4.556
5 year journal impact factor 4.653
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