authors |
Sousa, AR; Martins-Cruz, C; Oliveira, MB; Mano, JF |
nationality |
International |
journal |
ADVANCED MATERIALS |
author keywords |
biomaterial-free constructs; cell therapies; fiber-shaped tissue; scaffold-free cellular aggregates; tissue engineering |
keywords |
EXTRACELLULAR-MATRIX; ANGIOGENESIS; VASCULARIZATION; MORPHOGENESIS; SPHEROIDS; HYDROGELS; HEALTH |
abstract |
Cellular aggregates are used as relevant regenerative building blocks, tissue models, and cell delivery platforms. Biomaterial-free structures are often assembled either as 2D cell sheets or spherical microaggregates, both incompatible with free-form deposition, and dependent on challenging processes for macroscale 3D upscaling. The continuous and elongated nature of fiber-shaped materials enables their deposition in unrestricted multiple directions. Cellular fiber fabrication has often required exogenously provided support proteins and/or the use of biomaterial-based sacrificial templates. Here, the rapid (<24 h) assembly of fiberoids is reported: living centimeter-long scaffold-free fibers of cells produced in the absence of exogenous materials or supplements. Adipose-derived mesenchymal stem cell fiberoids can be easily modulated into complex multidimensional geometries and show tissue-invasive properties while keeping the secretion of trophic factors. Proangiogenic properties studied on a chick chorioallantoic membrane in an ovo model are observed for heterotypic fiberoids containing endothelial cells. These micro-to-macrotissues may find application as morphogenic therapeutic and tissue-mimetic building blocks, with the ability to integrate 3D and 4D full biological materials. |
publisher |
WILEY-V C H VERLAG GMBH |
issn |
0935-9648 |
isbn |
1521-4095 |
year published |
2020 |
volume |
32 |
issue |
2 |
digital object identifier (doi) |
10.1002/adma.201906305 |
web of science category |
Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter |
subject category |
Chemistry; Science & Technology - Other Topics; Materials Science; Physics |
unique article identifier |
WOS:000498465700001
|