The hydrophobic polysaccharides of apple pomace
authors Fernandes, PAR; Silva, AMS; Evtuguin, DV; Nunes, FM; Wessel, DF; Cardoso, SM; Coimbra, MA
nationality International
journal CARBOHYDRATE POLYMERS
author keywords Pectic polysaccharides; Xyloglucan; Arabinan; Polyphenol interaction; NMR
keywords RICH PECTIC POLYSACCHARIDES; CELL-WALLS; STRUCTURAL CHARACTERIZATION; PHENOLIC-COMPOUNDS; HAIRY REGIONS; ARABINAN; POLYPHENOLS; JUICE; NMR; OLIGOSACCHARIDES
abstract In this work, polysaccharides extracted with hot water from apple pomace were isolated by C18 cartridge solid-phase extraction at pH 7 (Fr7). Dialysis (12-14 kDa) of this fraction allowed to obtain 17% (w/w) of polymeric material composed by 65% of polysaccharides, mainly arabinose (58 mol%), galacturonic acid (16 mol%) and glucose (10 mol%). Folin-Ciocalteu assay showed 62 g of phloridzin equiv/kg of polyphenols. Moreover, adjusting to pH 3, it was possible to retain an additional fraction (Fr3) representing a further 4% of the polymeric material. Fr3 contained 53% of polysaccharides composed mainly by galacturonic acid (66 mol%) and polyphenols accounted for 37 g of phloridzin equiv/kg. Precipitation with ethanol and subsequent methylation and NMR spectroscopic analysis of Fr7 dialysate allowed the identification of covalently-linked pectic-polyphenol-xyloglucan and arabinan-polyphenol complexes. These structures are possibly formed as a result of polyphenol oxidation reactions during the industrial processing of apples, conferring hydrophobic characteristics to apple pomace polysaccharides.
publisher ELSEVIER SCI LTD
issn 0144-8617
year published 2019
volume 223
digital object identifier (doi) 10.1016/j.carbpol.2019.115132
web of science category Chemistry, Applied; Chemistry, Organic; Polymer Science
subject category Chemistry; Polymer Science
unique article identifier WOS:000481570700079
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journal impact factor 5.158
5 year journal impact factor 5.326
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