Pullulan microneedle patches for the efficient transdermal administration of insulin envisioning diabetes treatment
authors Fonseca, DFS; Costa, PC; Almeida, IF; Dias-Pereira, P; Correia-Sa, I; Bastos, V; Oliveira, H; Duarte-Araujo, M; Morato, M; Vilela, C; Silvestre, AJD; Freire, CSR
nationality International
journal CARBOHYDRATE POLYMERS
author keywords Pullulan; Polysaccharides; Insulin; Microneedles; Transdermal drug delivery
keywords POLYMER MICRONEEDLES; DELIVERY; NANOPARTICLES; FABRICATION; CELLULOSE; ALGINATE; RELEASE; FILMS; ACID
abstract The present study reports the fabrication of dissolvable microneedle (MN) patches using pullulan (PL), a watersoluble polysaccharide with excellent film-forming ability, for the transdermal administration of insulin, envisioning the non-invasive treatment of diabetes. PL MNs patches were successfully prepared by micromoulding and revealed good thermal stability (T-dmax = 294 degrees C) and mechanical properties (> 0.15 N needle(-1)), penetrating skin up to 381 mu m depth, as revealed by in vitro skin tests. After application into human abdominal skin in vitro, the MNs dissolved within 2 h releasing up to 87% of insulin. When stored at 4, 20 and 40 degrees C for 4 weeks, insulin was able to retain its secondary structure, as shown by circular dichroism spectropolarimetry. The prepared PL MNs were non-cytotoxic towards human keratinocytes, being suitable for skin application. These findings suggest that PL MNs have potential to deliver insulin transdermally, thus avoiding its subcutaneous administration.
publisher ELSEVIER SCI LTD
issn 0144-8617
isbn 1879-1344
year published 2020
volume 241
digital object identifier (doi) 10.1016/j.carbpol.2020.116314
web of science category Chemistry, Applied; Chemistry, Organic; Polymer Science
subject category Chemistry; Polymer Science
unique article identifier WOS:000539100600049
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  impact metrics
journal analysis (jcr 2019):
journal impact factor 7.182
5 year journal impact factor 6.89
category normalized journal impact factor percentile 96.638
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