Controlling the l-asparaginase extraction and purification by the appropriate selection of polymer/salt-based aqueous biphasic systems

authors	Magri, A; Pimenta, MV; Santos, JHPM; Coutinho, JAP; Ventura, SPM; Monteiro, G; Rangel-Yagui, CO; Pereira, JFB
nationality	International
journal	JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
author keywords	enzymes; aqueous biphasic systems (ABS); bioseparations; liquid-liquid extraction; purification; separation
keywords	2-PHASE SYSTEMS; IONIC LIQUIDS; RELATIVE EFFECTIVENESS; POLYETHYLENE-GLYCOL; PRIMARY RECOVERY; PROTEINS; PARTITION; BIOPHARMACEUTICALS; BIODEGRADABILITY; TETRACYCLINE
abstract	BACKGROUND l-Asparaginase (ASNase) is an important biopharmaceutical for the treatment of acute lymphoblastic leukemia (ALL); however, with some restrictions due to its high manufacturing costs. Aqueous biphasic systems (ABS) have been suggested as more economical platforms for the separation/purification of proteins, but a full understanding of the mechanisms behind the ASNase partition is still a major challenge. Polymer/salt-based ABS with different driving-forces (salting-out and hydrophilicity/hydrophobicity effects) were herein applied to control the partition of commercial ASNase. RESULTS The main results showed the ASNase partition to the salt- or polymer-rich phase depending on the ABS studied, with extraction efficiencies higher than 95%. For systems composed of inorganic salts, the ASNase partition was controlled by the polyethylene glycol (PEG) molecular weight used. Cholinium-salts-based ABS were able to promote a preferential ASNase partition to the polymer-rich phase using PEG-600 and to the salt-rich phase using a more hydrophobic polypropylene glycol (PPG)-400 polymer. It was possible to select the ABS composed of PEG-2000 + potassium phosphate buffer as the most efficient to separate the ASNase from the main contaminant proteins (purification factor = 2.4 +/- 0.2), while it was able to maintain the enzyme activity for posterior application as part of a therapeutic. CONCLUSION Polymer/salt ABS can be used to control the partition of ASNase and adjust its purification yields, demonstrating the ABS potential as more economic platform for the selective recovery of therapeutic enzymes from complex broths. (c) 2019 Society of Chemical Industry
publisher	WILEY
issn	0268-2575
isbn	1097-4660
year published	2020
volume	95
issue	4
beginning page	1016
ending page	1027
digital object identifier (doi)	10.1002/jctb.6281
web of science category	Biotechnology & Applied Microbiology; Chemistry, Multidisciplinary; Engineering, Environmental; Engineering, Chemical
subject category	Biotechnology & Applied Microbiology; Chemistry; Engineering
unique article identifier	WOS:000502600100001