Biosurfactant Production in Sub-Oxic Conditions Detected in Hydrocarbon-Degrading Isolates from Marine and Estuarine Sediments
authors Domingues, PM; Oliveira, V; Serafim, LS; Gomes, NCM; Cunha, A
nationality International
journal INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
author keywords deep-sea; estuary; anaerobic; atomized oil assay; petroleum hydrocarbons; anoxic
keywords POLYCYCLIC AROMATIC-HYDROCARBONS; COLD SEEP SEDIMENTS; DEEP-SEA SEDIMENTS; BACTERIAL COMMUNITIES; OIL-RECOVERY; PSEUDOMONAS-AERUGINOSA; REDUCING BACTERIA; SP NOV.; DIVERSITY; BIODEGRADATION
abstract Hydrocarbon bioremediation in anoxic sediment layers is still challenging not only because it involves metabolic pathways with lower energy yields but also because the production of biosurfactants that contribute to the dispersion of the pollutant is limited by oxygen availability. This work aims at screening populations of culturable hydrocarbonoclastic and biosurfactant (BSF) producing bacteria from deep sub-seafloor sediments (mud volcanos from Gulf of Cadiz) and estuarine sub-surface sediments (Ria de Aveiro) for strains with potential to operate in sub-oxic conditions. Isolates were retrieved from anaerobic selective cultures in which crude oil was provided as sole carbon source and different supplements were provided as electron acceptors. Twelve representative isolates were obtained from selective cultures with deep-sea and estuary sediments, six from each. These were identified by sequencing of 16S rRNA gene fragments belonging to Pseudomonas, Bacillus, Ochrobactrum, Brevundimonas, Psychrobacter, Staphylococcus, Marinobacter and Curtobacterium genera. BSF production by the isolates was tested by atomized oil assay, surface tension measurement and determination of the emulsification index. All isolates were able to produce BSFs under aerobic and anaerobic conditions, except for isolate DS27 which only produced BSF under aerobic conditions. These isolates presented potential to be applied in bioremediation or microbial enhanced oil recovery strategies under conditions of oxygen limitation. For the first time, members of Ochrobactrum, Brevundimonas, Psychrobacter, Staphylococcus, Marinobacter and Curtobacterium genera are described as anaerobic producers of BSFs.
publisher MDPI
year published 2020
volume 17
issue 5
digital object identifier (doi) 10.3390/ijerph17051746
web of science category Environmental Sciences; Public, Environmental & Occupational Health
subject category Environmental Sciences & Ecology; Public, Environmental & Occupational Health
unique article identifier WOS:000522389200286
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