Carbon nanostructure morphology templates nanocomposites for phosphoproteomics
authors Piovesana, S; Iglesias, D; Melle-Franco, M; Kralj, S; Cavaliere, C; Melchionna, M; Lagana, A; Capriotti, AL; Marchesan, S
nationality International
journal NANO RESEARCH
author keywords nanocomposites; carbon nanotubes; graphene oxide; carbon nanohorns; proteomics; magnetic
keywords HIGHLY SELECTIVE ENRICHMENT; METAL-OXIDES; FUNCTIONALIZATION; PHOSPHOPEPTIDES; COMPOSITE; BLACK
abstract Protein and peptide phosphorylation regulate numerous pathological processes, however, their characterization is challenging due to their low abundance and transient nature. Therefore, nanomaterials are being developed to address this demanding task. In particular, carbon nanostructures are attracting interest as scaffolds for functional nanocomposites, yet only isolated studies exist on the topic, and little is known on the effect of nanocarbon morphology on templating nanocomposites. In this work, we compared oxidized carbon nanotubes, graphene oxide, oxidized carbon nanohorns and oxidized graphitized carbon black, as scaffolds for magnetized nanocomposites. The nanomaterials were extensively characterized with experimental and in silico techniques. Next, they were applied to phosphopeptide enrichment from cancer cell lysates for NanoHPLC-MS/MS, with selectivity as high as nearly 90% and several-thousand identification hits. Overall, new insights emerged for the understanding and the design of nanocomposites for phosphoproteomics.
publisher TSINGHUA UNIV PRESS
issn 1998-0124
isbn 1998-0000
year published 2020
volume 13
issue 2
beginning page 380
ending page 388
digital object identifier (doi) 10.1007/s12274-020-2620-4
web of science category Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied
subject category Chemistry; Science & Technology - Other Topics; Materials Science; Physics
unique article identifier WOS:000519298200010
  ciceco authors
  impact metrics
journal analysis (jcr 2019):
journal impact factor 8.183
5 year journal impact factor 8.164
category normalized journal impact factor percentile 85.604
dimensions (citation analysis):
altmetrics (social interaction):



 


Sponsors

1suponsers_list_ciceco.jpg