Macrophage Metabolomics Reveals Differential Metabolic Responses to Subtoxic Levels of Silver Nanoparticles and Ionic Silver
authors Carrola, J; Bastos, V; Daniel-da-Silva, AL; Gil, AM; Santos, C; Oliveira, H; Duarte, IF
nationality International
journal EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
author keywords Macrophages; Silver; Nanoparticles; NMR metabolomics; Cell metabolism
keywords TOXICITY MECHANISM; SUCCINATE-DEHYDROGENASE; CELLS STRUCTURE; IN-VITRO; ITACONATE; HEXOKINASE; SIZE
abstract This study employed NMR metabolomics to characterize macrophage responses to subtoxic concentrations of silver nanoparticles (AgNPs ca. 30 nm) and ionic silver (Ag+), with a view to further elucidate their immunomodulatory activity at the cell metabolism level. Exposure to AgNPs caused RAW 264.7 macrophages to decrease intracellular glucose utilization, possibly due to interference with glycolytic enzymes, and to reprogram the TCA cycle towards anaplerotic fueling and production of anti-inflammatory metabolites (e.g. itaconate and creatine). Moreover, AgNPs-exposed cells were able to control the levels of reactive oxygen/nitrogen species (ROS/RNS), likely through upregulation of glutathione synthesis. On the other hand, macrophages exposed to Ag+ at equivalent subtoxic concentrations showed reduced metabolic activity, lower ability to counterbalance ROS/RNS and alterations in membrane-related lipids. Overall, the metabolomics approach hereby employed provided novel insights into the differential effects of AgNPs and Ag+, which help explain the lower toxic potential of nanosilver than silver ions.
publisher WILEY-V C H VERLAG GMBH
issn 1434-1948
isbn 1099-0682
year published 2020
volume 2020
issue 19
beginning page 1867
ending page 1876
digital object identifier (doi) 10.1002/ejic.202000095
web of science category Chemistry, Inorganic & Nuclear
subject category Chemistry
unique article identifier WOS:000530644100001

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