Multiorgan histopathological changes in the juvenile seabreamSparus aurataas a biomarker for zinc oxide particles toxicity
authors Beegam, A; Lopes, M; Fernandes, T; Jose, J; Barreto, A; Oliveira, M; Soares, AMVM; Trindade, T; Thomas, S; Pereira, ML
nationality International
journal ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
author keywords Zinc oxide particles; Fish; Biomarkers; Histopathology; Acute exposure
keywords SPARUS-AURATA; SUBLETHAL CONCENTRATION; COPPER NANOPARTICLES; ZNO NANOPARTICLES; FISH; GILLS; LIVER; LAGOON; CONTAMINATION; INDICATORS
abstract Zinc oxide nanoparticles are widely used in some domains (cosmetics, pharmaceuticals optical devices, and agricultural field) due to their physical, optical, and antimicrobial properties. However, the release of ZnO-NPs into the environment may affect organisms like fish with potential consequences for human health. Histological approaches of the acute effects of these materials on fish are scarce; thus, the present study aimed to investigate the potential toxic effects of acute exposure to ZnO particles in marine environments, by assessing histological changes in the gills, liver, spleen, and muscle of gilthead seabream (Sparus aurata) juveniles. Thus, fish were exposed for 96 h, via water, to 1 mg L(-1)of ionic zinc and zinc oxide particles (1.1, 1.2, and 1.4 mu m of size). Histological examination revealed gills as the most affected organ, followed by liver, muscle, and spleen. In the gills, histopathological changes included hyperplasia of epithelial cells, fusion of the secondary lamellae, and lifting of the lamellar epithelium with edema. In the liver, lipid vacuolation of several degrees, necrosis of hepatic and pancreatic tissues, blood congestion in sinusoids and hepatoportal vessels, presence of cellular infiltrate, and melano-macrophages diffusion was found. Muscle showed degeneration, atrophy, thickening and necrosis of muscle fibers with edema between them, and presence of melano-macrophages in the muscle layer. Spleen was the less damaged organ, displaying congested blood, white pulp increase/rupture, and bigger and darker melano-macrophage aggregates in the splenic stroma. These results underline that the size of particles plays a determinant role in their potential pernicious effects. A short-term exposure caused major histopathological changes in relevant organs ofS. auratajuveniles, possibly affecting their function.
publisher SPRINGER HEIDELBERG
issn 0944-1344
isbn 1614-7499
year published 2020
volume 27
issue 25
beginning page 30907
ending page 30917
digital object identifier (doi) 10.1007/s11356-019-05949-7
web of science category Environmental Sciences
subject category Environmental Sciences & Ecology
unique article identifier WOS:000554795000002
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