Real-Time Intracellular Temperature Imaging Using Lanthanide Bearing Polymeric Micelles
authors Pinol, R; Zeler, J; Brites, CDS; Gu, YY; Tellez, P; Neto, ANC; da Silva, TE; Moreno-Loshuertos, R; Fernandez-Silva, P; Gallego, AI; Martinez-Lostao, L; Martinez, A; Carlos, LD; Millan, A
nationality International
journal NANO LETTERS
author keywords Intracellular temperature mapping; Luminescence thermometry; Thermogenesis; Lanthanide-based polymer nanomicelles
keywords ION ENERGY-TRANSFER; FLUORESCENT THERMOSENSORS; COORDINATION-COMPOUNDS; GAP ISSUE; THERMOMETRY; SENSORS
abstract Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln(3+)-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to similar to 20 degrees and subcellular gradients of similar to 5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes.
publisher AMER CHEMICAL SOC
issn 1530-6984
isbn 1530-6992
year published 2020
volume 20
issue 9
beginning page 6466
ending page 6472
digital object identifier (doi) 10.1021/acs.nanolett.0c02163
web of science category Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter
subject category Chemistry; Science & Technology - Other Topics; Materials Science; Physics
unique article identifier WOS:000571442000031
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journal impact factor 11.238
5 year journal impact factor 12.727
category normalized journal impact factor percentile 89.991
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