Potential of NMR Spectroscopy for the study of human amniotic fluid
authors Graca, G; Duarte, IF; Goodfellow, BJ; Barros, AS; Carreira, IM; Couceiro, AB; Spraul, M; Gil, AM
nationality International
journal ANALYTICAL CHEMISTRY
keywords MAGNETIC-RESONANCE-SPECTROSCOPY; RESOLUTION H-1-NMR SPECTROSCOPY; DIFFUSION-ORDERED SPECTROSCOPY; P-31 NMR; PLASMA; H-1; HEPATOTOXICITY; METABONOMICS; BIOFLUIDS; STORAGE
abstract 1D, and 2D 800 MHz high-resolution nuclear magnetic resonance spectroscopy of human amniotic fluid (HAF) enabled the identification of similar to 50 metabolites. In addition, liquid chromatography-NMR and diffusion ordered spectroscopy (DOSY) allowed signal overlap to be reduced and the characterization of higher molecular weight (M-w) components, respectively. Indeed, the DOSY spectrum of,a M-w >10 kDa HAF fraction enabled three protein families, differing in average Mw, to be detected and may therefore be of potential value in the study of disorderrelated variations in HAF protein profiles. The effects of freeze-drying, storage at -20 or -70 degrees C, and freeze-thawing cycles on HAF compositional stability were investigated, as well as stability at room temperature (to account for overnight data acquisition runs). These data are the basis for establishing statistically validated correlations between HAF NMR data and any physiological disorders of the fetus/mother. Freeze-drying caused signal loss for urea, ethanol, and compounds resonating at 2.22 and 1.17 ppm. Storage at -70 degrees C or lower is recommended since only minor compositional changes were observed, affecting mainly acetate and pyruvate. Freeze-thaw cycles did not cause significant compositional changes, and room-temperature stability studies indicated a 4-5 h maximum period of handling/ acquisition time to ensure HAF stability.
publisher AMER CHEMICAL SOC
issn 0003-2700
year published 2007
volume 79
issue 21
beginning page 8367
ending page 8375
digital object identifier (doi) 10.1021/ac071278d
web of science category Chemistry, Analytical
subject category Chemistry
unique article identifier WOS:000250584800060
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