Influence of Spray-dried Hydroxyapatite-5-Fluorouracil Granules on Cell Lines Derived from Tissues of Mesenchymal Origin
authors Scharnweber, T; Santos, C; Franke, RP; Almeida, MM; Costa, MEV
nationality International
journal MOLECULES
author keywords Hydroxyapatite; Spray dried particles; 5-Fluorouracil; in Vitro cytotoxicity; Drug delivery system
keywords BASIC CALCIUM-PHOSPHATE; HYDROXYAPATITE NANOPARTICLES; CONTROLLED MORPHOLOGY; PHOSPHOCITRATE; MITOGENESIS; MECHANISMS; PARTICLES; APOPTOSIS; DELIVERY; BEHAVIOR
abstract In our previous work we described the preparation and characterization of spray dried hydroxyapatite micro granules loaded with 5-fluorouracil (5-FU). These loaded particles are used as a model drug delivery system (DDS). In this study we examined the in vitro response of two cell lines derived from different tissues to 5-FU loaded granules (LG). Both cell lines, either L929 cells of a mouse fibroblast lineage or cells originating from a rat osteosarcoma (ROS 17/2.8) showed a dose dependent decrease in cell proliferation in response to 5-FU-, either dissolved in the culture medium or loaded onto particles. The response of the two cell lines to loaded and nonloaded particles was different. The effect of LG and of a corresponding concentration of free 5-FU was practically the same for the ROS 17/2.8 cells indicating that ROS 17/2.8 cells were not affected by the carrier material. In contrast, L929 cells showed a slight decrease in cell proliferation also in the presence of granules not loaded with 5-FU. This is thought to be attributed to the inhibition of mitogenesis by phosphocitrates, already demonstrated in fibroblasts. In summary, we found that the loaded 5-FU kept its effectivity after the spray drying process and that the response towards the granules varied with cell type. This is the first step towards a tissue specific DDS.
publisher MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL-MDPI
issn 1420-3049
year published 2008
volume 13
issue 11
beginning page 2729
ending page 2739
digital object identifier (doi) 10.3390/molecules13112729
web of science category Chemistry, Organic
subject category Chemistry
unique article identifier WOS:000261198000003
  ciceco authors
  impact metrics
times cited (wos core): 7
journal impact factor (jcr 2016): 2.861
5 year journal impact factor (jcr 2016): 2.988
category normalized journal impact factor percentile (jcr 2016): 72.034
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