Preliminary structural characterization of human SOUL, a haem-binding protein
authors Freire, F; Romao, MJ; Macedo, AL; Aveiro, SS; Goodfellow, BJ; Carvalho, AL
nationality International
journal ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
keywords MOLECULAR-REPLACEMENT; P22HBP; FAMILY
abstract Human SOUL (hSOUL) is a 23 kDa haem-binding protein that was first identified as the PP(23) protein isolated from human full-term placentas. Here, the overexpression, purification and crystallization of hSOUL are reported. The crystals belonged to space group P6(4)22, with unit-cell parameters a = b = 145, c = 60 angstrom and one protein molecule in the asymmetric unit. X-ray diffraction data were collected to 3.5 angstrom resolution at the ESRF. A preliminary model of the three-dimensional structure of hSOUL was obtained by molecular replacement using the structures of murine p22HBP (PDB codes 2gov and 2hva), obtained by solution NMR, as search models.
publisher WILEY-BLACKWELL PUBLISHING, INC
issn 1744-3091
year published 2009
volume 65
beginning page 723
ending page 726
digital object identifier (doi) 10.1107/S174430910902291X
web of science category Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography
subject category Biochemistry & Molecular Biology; Biophysics; Crystallography
unique article identifier WOS:000267530000018
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