Design of ionic liquids for lipase purification
authors Ventura, SPM; Sousa, SG; Freire, MG; Serafim, LS; Lima, AS; Coutinho, JAP
nationality International
journal JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
author keywords Candida antarctica lipase B; Ionic liquid; Purification process; Partition process
keywords AQUEOUS BIPHASIC SYSTEMS; 2-PHASE SYSTEMS; PARTITIONING BEHAVIOR; MUTUAL SOLUBILITIES; POLYETHYLENE-GLYCOL; SELF-AGGREGATION; PENICILLIN-G; EXTRACTION; PROTEINS; SALT
abstract Aqueous two-phase systems (ATPS) are considered as efficient downstream processing techniques in the production and purification of enzymes, since they can be considered harmless to biomolecules due to their high water content and due to the possibility of maintaining a neutral pH value in the medium. A recent type of alternative ATPS is based on hydrophilic ionic liquids (ILs) and salting-out inducing salts. The aim of this work was to study the lipase (Candida antarctica lipase B - CaLB) partitioning in several ATPS composed of ionic liquids (ILs) and inorganic salts, and to identify the best IL for the enzyme purification. For that purpose a wide range of IL cations and anions, and some of their combinations were studied. For each system the enzyme partitioning between the two phases was measured and the purification factors and enzyme recoveries were determined. The results indicate that the lipase maximum purification and recovery were obtained for cations with a C-8 side alkyl chain, the [N(CN)(2)] anion and Its belonging to the pyridinium family. However, the highest purification parameters were observed for 1-methyl-3-octylimidazolium chloride [C(8)mim]Cl, suggesting that the IL extraction capability does not result from a cumulative character of the individual characteristics of ILs. The results indicate that the IL based ATPS have an improved performance in the lipase purification and recovery. (C) 2011 Elsevier B.V. All rights reserved.
publisher ELSEVIER SCIENCE BV
issn 1570-0232
year published 2011
volume 879
issue 26
beginning page 2679
ending page 2687
digital object identifier (doi) 10.1016/j.jchromb.2011.07.022
web of science category Biochemical Research Methods; Chemistry, Analytical
subject category Biochemistry & Molecular Biology; Chemistry
unique article identifier WOS:000295297800001
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