Simple screening method to identify toxic/non-toxic ionic liquids: Agar diffusion test adaptation
authors Ventura, SPM; de Barros, RLF; Sintra, T; Soares, CMF; Lima, AS; Coutinho, JAP
nationality International
journal ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
author keywords Antimicrobial activity; Ionic liquids; Bacteria; Yeast; Mold; Morphologic characteristics
keywords ANTIMICROBIAL ACTIVITIES; PARTITION-COEFFICIENTS; ORGANIC-MATTER; IMIDAZOLIUM; LIPOPHILICITY
abstract A wide range of ionic liquids (ILs), containing a diverse set of cations, anions and alkyl chain lengths, was screened for their antimicrobial activity toward four microorganisms, Escherichia coli CCT-0355, Staphylococcus aureus ATCC-6533, Fusarium sp. LM03 and Candida albicans ATCC-76645. For that purpose an adaptation of the Agar Diffusion test was validated and successfully applied as a rapid screen method to identify toxic ILs, avoiding the use of more complex and expensive techniques. The effects of the cation alkyl chain length were studied, being observed both the "alkyl side chain" effect (increase in antimicrobial activity with the elongation of the alkyl chain) and "cut-off" effect (beyond a given chain length, the toxicity cannot be increased any further). lmidazolium-based ILs have in general, negative effects on the growth of these microorganisms dependent on the anion and alkyl chain length (growth inhibition halo from 1.98 +/- 0.04 mm for [C(2)mim]Cl to 39.53 +/- 0.81 mm for [C(10)mim]Cl). On the opposite, the phosphonium-based ILs do not seem to have negative effects for the longest alkyl chains (growth inhibition halos between 0.00 +/- 0.00 and 7.30 +/- 0.42 mm). It was also observed that the alkyl chain, cation family, and anion moiety all have significant effects on the antimicrobial activity these effects being well correlated with the lipophilicity of the ILs tested. The results also show that the microorganisms responses to the diverse ILs tested are dependent on their morphologic differences. (c) 2012 Elsevier Inc. All rights reserved.
publisher ACADEMIC PRESS INC ELSEVIER SCIENCE
issn 0147-6513
year published 2012
volume 83
beginning page 55
ending page 62
digital object identifier (doi) 10.1016/j.ecoenv.2012.06.002
web of science category Environmental Sciences; Toxicology
subject category Environmental Sciences & Ecology; Toxicology
unique article identifier WOS:000307129700008
  ciceco authors
  impact metrics
journal analysis (jcr 2017):
journal impact factor 3.974
5 year journal impact factor 4.000
category normalized journal impact factor percentile 81.528
dimensions (citation analysis):
altmetrics (social interaction):



 


Sponsors

1suponsers_list_ciceco.jpg