Supercritical Fluid Extraction of Eucalyptus globulus Bark-A Promising Approach for Triterpenoid Production
authors Domingues, RMA; Oliveira, ELG; Freire, CSR; Couto, RM; Simoes, PC; Neto, CP; Silvestre, AJD; Silva, CM
nationality International
journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
author keywords Eucalyptus globulus; bark; triterpenoids; triterpenic acids; supercritical fluid extraction; carbon dioxide; biorefinery
keywords CHROMATOGRAPHY-MASS SPECTROMETRY; BIOACTIVE PHENOLIC SUBSTANCES; LIPOPHILIC EXTRACTIVES; CHEMICAL-COMPOSITION; GAS-CHROMATOGRAPHY; CARBON-DIOXIDE; OUTER BARKS; PHYTOSTEROLS; FUTURE; BIOREFINERIES
abstract Eucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids). In this work, the supercritical fluid extraction (SFE) of Eucalyptus globulus deciduous bark was carried out with pure and modified carbon dioxide to recover this fraction, and the results were compared with those obtained by Soxhlet extraction with dichloromethane. The effects of pressure (100-200 bar), co-solvent (ethanol) content (0, 5 and 8% wt), and multistep operation were studied in order to evaluate the applicability of SFE for their selective and efficient production. The individual extraction curves of the main families of compounds were measured, and the extracts analyzed by GC-MS. Results pointed out the influence of pressure and the important role played by the co-solvent. Ethanol can be used with advantage, since its effect is more important than increasing pressure by several tens of bar. At 160 bar and 40 degrees C, the introduction of 8% (wt) of ethanol greatly improves the yield of triterpenoids more than threefold.
publisher MDPI AG
issn 1422-0067
year published 2012
volume 13
issue 6
beginning page 7648
ending page 7662
digital object identifier (doi) 10.3390/ijms13067648
web of science category Biochemistry & Molecular Biology; Chemistry, Multidisciplinary
subject category Biochemistry & Molecular Biology; Chemistry
unique article identifier WOS:000306188700072
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journal impact factor 3.687
5 year journal impact factor 3.878
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